Gelayee Dessalegn Asmelashe, Mohammedbrhan Abdelwuhab, Assefa Belay Asrie
Ruta chalepensis has been used in the Eastern Africa for management of malaria despite lack of scientific evaluations. This study was carried out to evaluate the in vivo antiplasmodial activity of the 80% methanol extract of the leaves of the plant. The screening was done using Swiss albino mice against chloroquine sensitive rodent malaria parasite, Plasmodium berghei, following the standard 4-day suppressive test procedure. Three extract doses (100,200 and 400 mg/kg/day) were given orally. Parasitemia level, survival time as well as weight variation were measured for evaluating antiplasmodial efficacy. Moreover, preliminary phytochemical and acute toxicity studies were carried out.The presence of alkaloids, flavonoids, polyphenols, terpenoids, cardiac glycosides as well as saponins was confirmed. The oral LD50 was determined to be higher than 2000mg/kg. Antiplamodial activity of the extract was inversely related with the dose so that the100mg/kg dose was the most effective (mean parasitemia of 29.92 +0.94 on day 4, P<0.001). Ruta chalepensis therefore has antimalarial activity and no significant acute toxicity when taken orally. The study validates the traditional use of the plant in managing malaria. We suggest further investigation on this potential plant to address the call for a novel antimalarial drug.
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Md. Saddam Hussain, Mohammad Salim Hossain, Niloy Sen, Md. Abdur Rahman, Md. Abdul Halim
Different concentration of methanolic extracts of seeds of Vigna unguiculata (cowpea) Linn collected from the local area of Noakhali, Bangladesh were studied for membrane Stabilizing Assay. V.unguiculata Linn seeds were initially collected, processed and extracted with methanol. Then, five different concentration (1mg/ml, 3mg/ml, 5mg/ml, 7mg/ml, 9mg/ml) of methanolic extract of Cowpea (V.unguiculata) were subjected for determination of membrane stabilizing activity. In the assay for membrane stabilizing activity, five different concentration of crude methanol extract capable to inhibit hemolysis of erythrocyte membrane dose dependently in hypotonic solution and heat- induced conditions, which indicates the anti-inflammatory property of the samples. Where, Acetyl salicylic acid was used as standard drug. From the above discussion it is clear that Vigna unguiculata Linn seeds methanolic extract showed significant anti-inflammatory potentials. So, it will be very much possible source for isolating lead compound for curing inflammatory disorder.
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