Chromolaena odorata is widely used for wound healing in Bangladesh. The present study was an attempt to evaluate the antioxidant, cytotoxic and anthelmintic properties of crude methanolic extract of C. odorata and different fractions of the crude extract. The phytochemical screening revealed the potent source of different phytochemical constituents including Alkaloids, Phytosterols, Tannins, Terpenes, Fats and Fixed oils. Determination of total phenolic contents and DPPH methods were evaluated for antioxidant activity. The total phenolic content (mg of GAE / gm) of Crude Methanol Extract (CME), Petroleum Ether Soluble Fraction (PESF), Ethyl Acetate Soluble Fraction (EASF) and Chloroform Soluble Fraction (CSF) were 71.08±0.38, 52.08±1.01, 86.33±0.38 and 54.25±0.90, respectively. In case of DPPH method, the IC₅₀ values were 19.48±0.49 µg/ml, 22.44±0.63µg/ml, 99.05±0.81µg/ml, 166.96±0.51 µg/ml, 317.10±0.99 µg/ml for standard (BHT: tert-butyl-1-hydroxytoluene) and the four extracts, respectively. Moreover brine shrimp lethality bioassay method was applied to determine the cytotoxic  activity. The LC₅₀ values were 0.839 µg/ml, 10.245 µg/ml, 8.98 µg/m, 8.28 µg/ml and 9.298 µg/ml for standard group (vincristin sulphate),  CME, PESF, EASF and CSF respectively in brine shrimp lethality bioassay. Besides, this study was also undertaken to evaluate anthelmintic activity (using Pheretima posthuma model) where albendazole was used as reference standard. For the this test, paralysis and death time for standard (albendazole) 10mg/ml and crude extracts (10, 20, 30, 40, 50) mg/ml are 56.20±0.20 and 77.4±0.24, 30.4±0.75 and 60.8±1.03, 24.6±0.32 and 50.2±0.37, 20.6±0.40 and 40.8±0.51, 16.4±0.68 and 32.4±1.29, 10.2±0.40 and 10.2±0.40 minutes respectively. From the study it was revealed that all the extracts were abundant of various phytochemicals and showed various biological activities. So, further studies are suggested to isolate the active constituents responsible for the activities and also to know the mechanism of actions.

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