Skip to main content Skip to main navigation menu Skip to site footer

Macaranga barteri stem bark extract exerts anti-inflammatory and anti-hyperalgesia activity in murine models



Abstract

This study was undertaken to evaluate the anti-inflammatory, anti-hyperalgesia and antioxidant activity of the hydro-alcoholic stem bark extract of Macaranga barteri (MBE). The carrageenan-induced foot oedema and Hargreaves thermal hyperalgesia models in rats were used to examine the anti-inflammatory and anti-hyperalgesic effects respectively. The 2, 2-diphenyl-2-picrylhydrazyl hydrate (DPPH) free radical scavenging and total antioxidant capacity assays were used to determine the antioxidant activity. In a curative protocol, MBE (30, 100, 300 mg kg-1, p.o.) dose dependently and significantly inhibited carrageenan-induced foot oedema by 37.01 ± 13.08, 53.01 ± 9.87 and 64.11 ± 9.05% respectively (ED50 = 89.37 ± 7.52 mg kg-1). The extract further attenuated cutaneous hyperalgesia by prolonging paw withdrawal latencies towards an external heat stimulus with an ED50 of 105.5 ± 4.22 mg kg-1. MBE was found to possess a total antioxidant capacity of 531.62 ± 10.98 mg g-1 dry weight (Gallic acid equivalent) and scavenged DPPH free radicals with an IC50 of 19.45 ± 1.46 µg/mL. The results provide the first report on the anti-inflammatory, analgesic and antioxidant activity of M. barteri stem bark and partly justify its traditional use in the management of inflammation and pain. This could be attributed to phytochemicals such as tannins, terpenoids, sterols, coumarins and flavonoids which were identified in preliminary phytochemical studies of the stem bark.

Keywords: Macaranga barteri inflammation analgesia Hargreaves carrageenan antioxidant

References

  1. Allison D, Ditor D. Immune dysfunction and chronic inflammation following spinal cord injury. Spinal Cord. 2015; 53(1):14-18.
  2. Fürst R,Zündorf I. Plant-derived anti-inflammatory compounds: hopes and disappointments regarding the translation of preclinical knowledge into clinical progress. Mediators Inflamm. 2014; 2014:1-9 http://dx.doi.org/10.1155/2014/146832.
  3. Stark TD, Mtui DJ, Balemba OB. Ethnopharmacological Survey of Plants Used in the Traditional Treatment of Gastrointestinal Pain, Inflammation and Diarrhea in Africa: Future Perspectives for Integration into Modern Medicine. Animals. 2013; 3(1):158-227 http://dx.doi.org/10.3390/ani3010158.
  4. Burkill HM, The useful plants of west tropical Africa. Volume 2: Families E-I. Kew: Royal Botanic Gardens; 1994.
  5. Magadula JJ. Phytochemistry and pharmacology of the genus Macaranga: a review. J Med Plants Res. 2014; 8(12):489-503 http://dx.doi.org/10.5897/JMPR2014.5396 .
  6. Herve EE, Bernard GN, Leandre KK, Paul YA, Etienne EE. Acute toxicity and gastric anti-ulcer activity of an aqueous extract of the leaves of Macaranga barteri Müll. Arg (Euphorbiaceae) on rat models. J Med Plants Res. 2018; 12(9):96-105 http://dx.doi.org/10.5897/JMPR2017.6547.
  7. Ogundajo A, Okeleye B, Ashafa AO. Chemical constituents, in vitro antimicrobial and cytotoxic potentials of the extracts from Macaranga barteri Mull-Arg. Asian Pac J Trop Biomed. 2017; 7(7):654-659 https://doi.org/10.1080/0035919X.2018.1552213
  8. Ogundajo AL,Tom Ashafa AO. Chemical profiling, antioxidant and carbohydrate-metabolising enzymes inhibitory potential of fractions from the leaves of Macaranga bateri Mull-Arg. Transactions of the Royal Society of South Africa. 2019; 1-11 https://doi.org/10.1080/0035919X.2018.1552213.
  9. Ngoumfo RM, Ngounou GE, Tchamadeu CV, Qadir MI, Mbazoa CD, Begum A, et al. Inhibitory effect of macabarterin, a polyoxygenated ellagitannin from Macaranga barteri, on human neutrophil respiratory burst activity. J Nat Pro. 2008; 71(11):1906-1910 https://doi.org/10.1021/np8004634.
  10. Evans WC, Trease and Evans' Pharmacognosy E-Book. Elsevier Health Sciences; 2009.
  11. Abotsi WKM, Lamptey SB, Afrane S, Boakye-Gyasi E, Umoh RU, Woode E. An evaluation of the anti-inflammatory, antipyretic and analgesic effects of hydroethanol leaf extract of Albizia zygia in animal models. Pharm Biol. 2017; 55(1):338-348 https://doi.org/10.1080/13880209.2016.1262434.
  12. Essel LB, Obiri DD, Osafo N, Antwi AO, Duduyemi BM. The Ethanolic Stem-Bark Extract of Antrocaryon micraster Inhibits Carrageenan-Induced Pleurisy and Pedal Oedema in Murine Models of Inflammation. ISRN. 2017; 2017:1-11 https://doi.org/10.1155/2017/6859230.
  13. Woode E, Ameyaw EO, Boakye-Gyasi E, Abotsi WK. Analgesic effects of an ethanol extract of the fruits of Xylopia aethiopica (Dunal) A. Rich (Annonaceae) and the major constituent, xylopic acid in murine models. J Pharm Bioall Sci. 2012; 4(4):291-301 https://doi.org/10.4103/0975-7406.103251.
  14. Chen Z, Bertin R, Froldi G. EC50 estimation of antioxidant activity in DPPH assay using several statistical programs. Food Chem. 2013; 138(1):414-20 http://dx.doi.org/10.1016/j.foodchem.2012.11.001 .
  15. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999; 269(2):337-41.
  16. Necas J,Bartosikova L. Carrageenan: a review. Veterinarni Medicina. 2013; 58(4):187-205.
  17. Hargreaves K, Dubner R, Brown F, Flores C, Joris J. A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia. Pain. 1988; 32(1):77-88 https://doi.org/10.1016/0304-3959(88)90026-7.
  18. Chou TC. Antiâ€inflammatory and analgesic effects of paeonol in carrageenanâ€evoked thermal hyperalgesia. Br J Pharmacol. 2003; 139(6):1146-52 https://doi.org/10.1038/sj.bjp.0705360.
  19. Balayssac D, Ling B, Ferrier J, Pereira B, Eschalier A, Authier N. Assessment of thermal sensitivity in rats using the thermal place preference test: description and application in the study of oxaliplatin-induced acute thermal hypersensitivity and inflammatory pain models. Behav Pharmacol. 2014; 25(2):99-111 https://doi.org/10.1097/FBP.0000000000000026 .
  20. Mansouri MT, Hemmati AA, Naghizadeh B, Mard SA, Rezaie A, Ghorbanzadeh B. A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats. Indian J Pharmacol. 2015; 47(3):292-298 https://doi.org/10.4103/0253-7613.157127.
  21. Ding Z, Dai Y, Hao H, Pan R, Yao X, Wang Z. Anti-inflammatory effects of scopoletin and underlying mechanisms. Pharm. Biol. 2008; 46(12):854-60 https://doi.org/10.1080/13880200802367155.
  22. Adesegun S, Elechi N, Coker H. Antioxidant power of Macaranga barteri leaf. Am. J. Food Technol. 2007; 2(6):543-49 https://doi.org/10.3923/ajft.2007.543.549.
  23. Hasanat A, Chowdhury T, Kabir M, Chowdhury M, Chy M, Barua J, et al. Antinociceptive Activity of Macaranga denticulata Muell. Arg.(Family: Euphorbiaceae): In Vivo and In Silico Studies. Medicines. 2017; 4(88):1-8 https://doi.org/10.3390/medicines4040088.
  24. Jang DS, Cuendet M, Hawthorne ME, Kardono LB, Kawanishi K, Fong HH, et al. Prenylated flavonoids of the leaves of Macaranga conifera with inhibitory activity against cyclooxygenase-2. Phytochemistry. 2002; 61(7):867-72 https://doi.org/10.1016/S0031-9422(02)00378-3.
  25. Gandhimathi R. Evaluation of anti-inflammatory activity of Macaranga peltata ROXB. J. Pharm. Biol. 2013; 3:36-39.

How to Cite

Asante-Kwatia, E., Jibira, Y., Mensah, A. Y., & Osei-Sarfoh, D. (2019). Macaranga barteri stem bark extract exerts anti-inflammatory and anti-hyperalgesia activity in murine models. Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 6(3). https://doi.org/10.15562/phytomedicine.2019.104
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

HTML
49

Total
18

Share