ORIGINAL ARTICLE

Effect of Asparagus racemosus on selected female reproductive parameters using Wistar rat model.

Linet Mwende Kaaria

Linet Mwende Kaaria
University of Nairobi. Email: linetmwee@gmail.com
Online First: October 15, 2019 | Cite this Article
Kaaria, L. 2019. Effect of Asparagus racemosus on selected female reproductive parameters using Wistar rat model.. Discovery Phytomedicine 6(4). DOI:10.15562/phytomedicine.2019.110


Dysmenorrhea is a major cause of female morbidity globally. Asparagus racemosus (ASP) is traditionally used to manage dysmenorrhea in Nakuru, Kenya. This study evaluates the effect of Asparagus racemosus on female reproductive parameters; estrus cyclicity, mating success, gestation length, litter size and dysmenorrhea. The effect of Asparagus racemosus and ibuprofen on isolated uterine strips was evaluated using six non-pregnant rats. The uterine strips were exposed to serial extract concentrations (20, 40, 80,160 mg/ml) and 20 mg/ml ibuprofen was used as a positive control.

There was significant increase of proestrus phase (P < 0.001) and a subsequent significant reduction in the metestrus (P < 0.01) and diestrus (P < 0.05). No significant difference in mating success and gestation length, however Ibuprofen caused significant disruption of gestation length (P < 0.05).  The treated groups produced higher number of pups compared to controls. The plant extract caused a dose dependent significant reduction in uterine force of contraction by (-0.15%, -5.13%, -7.97%, -19.55 %) at 20, 40, 80 and 160 mg/ml respectively. The plant extract also caused a significant decline in frequency of uterine contraction (-5.99%; -9.61%; -16.76% and -25.21%) The extract caused no mortality even at the limit dose; 5000 mg/kg. Asparagus racemosus reduces uterine force and frequency of contraction. This is probably the reason for its traditional use in dysmenorrhea management.

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References

Ozgoli G, Goli M, Moattar F. Comparison of effects of ginger, mefenamic acid, and ibuprofen on pain in women with primary dysmenorrhea. The journal of alternative and complementary medicine. 2009 Feb 1;15(2):129-32.

Patel V, Tanksale V, Sahasrabhojanee M, Gupte S, Nevrekar P. The burden and determinants of dysmenorrhoea: a population‐based survey of 2262 women in Goa, India. BJOG: An International Journal of Obstetrics & Gynaecology. 2006 Apr;113(4):453-63.

Proctor M, Farquhar C. Diagnosis and management of dysmenorrhoea. Bmj. 2006 May 11;332(7550):1134-8.

Wong LP. Attitudes towards dysmenorrhoea, impact and treatment seeking among adolescent girls: A rural school‐based survey. Australian Journal of Rural Health. 2011 Aug;19(4):218-23.

De Sanctis V, Soliman AT, Elsedfy H, Soliman NA, Soliman R, El Kholy M. Dysmenorrhea in adolescents and young adults: a review in different country. Acta Bio Medica Atenei Parmensis. 2016;87(3):233-46.

Allen LM, Lam AC. Premenstrual syndrome and dysmenorrhea in adolescents. Adolescent medicine: state of the art reviews. 2012 Apr;23(1):139-63.

Dawood MY. Dysmenorrhea and prostaglandins. InGynecologic endocrinology 1987 (pp. 405-421). Springer, Boston, MA.

Proctor ML, Roberts H, Farquhar CM. Combined oral contraceptive pill (OCP) as treatment for primary dysmenorrhoea. The Cochrane Database of Systematic Reviews. 2001(4):CD002120-.

Shaha P, Bellankimath A. Pharmacological Profile of Asparagus racemosus: A Review. Int. J. Curr. Microbiol. App. Sci. 2017;6(11):1215-23.

Joshi RK. Asparagus racemosus (Shatawari), phytoconstituents and medicinal importance, future source of economy by cultivation in Uttrakhand: A review. Inter. J. Herb. Med. 2016;4(4):18-21.

Kinage P, Chaudhari D. “Shatavari: one solution for various female health issues” a review. World Journal of Pharmacy and Pharmaceutical Sciences. 2016;5(5):1105-4.

Periyasamy A, Mahalingam K. Phytochemical screening and antimicrobial activity from five Indian medicinal plants against human pathogens. Middle East Journal of Scientific Research. 2010;5(6):477-82.

Kaingu CK, Oduma JA, Kanui TI. Practices of traditional birth attendants in Machakos District, Kenya. Journal of ethnopharmacology. 2011 Sep 1;137(1):495-502.

Telefo PB, Tagne SR, Koona OE, Yemele DM, Tchouanguep FM. Effect of the aqueous extract of Justicia insularis T. Anders (Acanthaceae) on ovarian folliculogenesis and fertility of female rats. African Journal of Traditional, Complementary and Alternative Medicines. 2012;9(2):197-203.

Yousaf Z, Wang Y, Baydoun E. Phytochemistry and pharmacological studies on Solanum torvum Swartz. Journal of Applied Pharmaceutical Science. 2013 Apr;3(4):152-60.

Ndebia EJ, Kamgang R, Nkeh-ChungagAnye BN. Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum torvum (Solanaceae). African Journal of Traditional, Complementary and Alternative Medicines. 2007;4(2):240-4.

Khatami Sabzevar M, Haghighi AH, Askari R. The Effect of Short-term Use of Chamomile Essence on Muscle Soreness in Young Girls after an Exhaustive Exercise. Journal of Medicinal Plants. 2017 Jun 15;2(62):63-73.

Kumar MC, Udupa AL, Sammodavardhana K, Rathnakar UP, Shvetha U, Kodancha GP. Acute toxicity and diuretic studies of the roots of Asparagus racemosus Willd in rats. West Indian Medical Journal. 2010 Jan;59(1):03-6.

Satué K, Gardón JC. A review of the estrous cycle and the neuroendocrine mechanisms in the mare. J Steroids Horm Sci. 2013;4(2):115.

Johnson S, Jones R. Costs and comparisons of estrous synchronization systems. InApplied Reproductive Strategies in Beef Cattle Workshop 2002 Sep 5 (p. 138).

Walmer DK, Wrona MA, Hughes CL, Nelson KG. Lactoferrin expression in the mouse reproductive tract during the natural estrous cycle: correlation with circulating estradiol and progesterone. Endocrinology. 1992 Sep 1;131(3):1458-66.

Patterson D, Forrest DW, Williams GL. Applied Reproductive Strategies in Beef Cattle. Texas FARMER Collection. 2007 Jan 3.

Kalia V, Jadav AN, Bhuttani KK. In vivo effect of Asparagus racemosus on serum gonadotrophin levels in immature female wistar rats. In2 nd World Congress of Biotechology Development of Herbal Medicine.–Lukhnow 2003.

Prasad N, Narmada B, Khanna S, Kulkarni KS. Evecare capsules in DUB. Obstetrics & Gynaecology Communications. 2002;3(3):51-3.

Kumar A, Singh A. Enhancement of conception rate by EveCare after ovulation induction by clomiphene citrate followed by intrauterine insemination. Advances in Obstetrics & Gynecology. 2001;1(5):283-5.

Wallace S, Keightley A, Gie C. Dysmenorrhoea. The Obstetrician & Gynaecologist. 2010 Jul;12(3):149-54.

Khulbe A. Asparagus racemosus (shatavari): a multipurpose plant. European Journal of Pharmaceutical and Medical Research. 2015;2:599-613.

Mishra J, Dash AK, Kumar S. Hundred Problems, One Solution Asparagus racemosus. World Journal of Pharmaceutical Research. 2013;3(1):201-11.

Bahmani M, Eftekhari Z, Jelodari M, Saki K, Abdollahi R, Majlesi M, Rafieian Kopaei M, Rasouli S. Effect of Iranian herbal medicines in dysmenorrhea phytotherapy. Journal of Chemical and Pharmaceutical Research. 2015(2):519-26.

Mirabi P, Alamolhoda SH, Esmaeilzadeh S, Mojab F. Effect of medicinal herbs on primary dysmenorrhoea-a systematic review. Iranian journal of pharmaceutical research: IJPR. 2014;13(3):757.

Shams Ardekani MR, Hadjiakhoondi A, Jamshidi AH, Abdi K. The study of volatile oil of Foeniculum vulgare Miller. In their tissue culture and comparison with the whole plant. Journal of Medicinal Plants. 2005 Sep 15;3(15):73-80.

Barene I, Daberte I, Zvirgzdina L, Iriste V. The complex technology on products of German chamomile. Medicina (Kaunas). 2003;39(2):127-31.


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