ORIGINAL ARTICLE

Phytochemical Screening and in vitro Antimicrobial Activity of Dracaena Afromontana Leaves

Jean NTAGANDA , Gratien Habarurema, Janvier Habinshuti, Adrien Rutikanga, Jean Bernard Ndayambaje

Jean NTAGANDA
. Email: drntaganda1@gmail.com

Gratien Habarurema


Janvier Habinshuti


Adrien Rutikanga


Jean Bernard Ndayambaje

Online First: January 15, 2020 | Cite this Article
NTAGANDA, J., Habarurema, G., Habinshuti, J., Rutikanga, A., Ndayambaje, J. 2020. Phytochemical Screening and in vitro Antimicrobial Activity of Dracaena Afromontana Leaves. Discovery Phytomedicine 7(1): 7-11. DOI:10.15562/phytomedicine.2020.112


Dracaena afromontana is a native species grown in the high mountain rainforest of Rwanda. This plant finds many traditionally medicinal uses in the treatment of chest pain, dermatitis, liver diseases and malaria. In this report, the dried Bay leaves were extracted with n-hexane, ethyl acetate and methanol by maceration giving 0.717 g (1.434%), 1.457 g (2.914%) and 6.319 g (12.64%) respectively. The extracts were concentrated for further phytochemical screening and evaluated for antimicrobial activity against E. coli and S. aureus using well diffusion method. In addition, the dracaena afromontana extracts were analyzed using thin layer chromatographic separation techniques leading to the identification and characterization of bioactive compounds including, terpenoids, flavonoids, tannins, phenols, saponins, reducing sugar and quinones. The results from TLC indicate that the higher retention factor (Rf2 = 0.77) was obtained via the use of ethyl acetate, whereas n-hexane gave a retention factor (Rf3 = 0.558). On the other hand, the methanol extract did not show any separation. The antimicrobial assay for the extracts was carried out using Lurial broth agar and Manitor salt over lay method. The findings of antimicrobial assay showed that methanolic extract of dracaena afromontana leaves has an antibacterial activity against the gram negative bacteria E.coli with the zone of inhibition of 16 mm, while the antibacterial activity of ethyl acetate extract against E. coli showed a smaller inhibition zone of 10 mm for diluted inoculums (10-2 and 10-1). For the gram positive bacteria S. aureus the inhibition zone by Dracaena afromontana was insignificant, whereby the methanolic and ethyl acetate extracts showed a maximum inhibition zone of 4 mm and 3 mm respectively. The n-hexane extract did not show any antibacterial activity against both pathogenic organisms.

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