ORIGINAL ARTICLE

Gastro-protective and anti-acidic effects of Corchorus trilocularis Linn against diclofenac-induced gastric ulcers.

CYRIL GEORGE SIRINGO , Paul mungai Mbugua, Boniface Mwangi Chege, Lincone linus Oluoch

CYRIL GEORGE SIRINGO
University of Nairobi. Email: cyrilsiringo@gmail.com

Paul mungai Mbugua
University of Nairobi

Boniface Mwangi Chege
DEDAN KIMATHI UNIVERSITY OF TECHNOLOGY

Lincone linus Oluoch
University of Nairobi
Online First: January 15, 2020 | Cite this Article
SIRINGO, C., Mbugua, P., Chege, B., Oluoch, L. 2020. Gastro-protective and anti-acidic effects of Corchorus trilocularis Linn against diclofenac-induced gastric ulcers.. Discovery Phytomedicine 7(1): 33-41. DOI:10.15562/phytomedicine.2020.116


Ethnopharmacological Relevance

Corchorus trilocularis Linn leaves are consumed as green leafy vegetables that are boiled and used as relish, or potherb by some rural communities in Eastern Africa for the treatment of stomach ulcers.

Aim: This study investigated the gastro-protective effects of Corchorus trilocularis Linn (local name ‘mrenda’), a popularly consumed vegetable in Kenya, on diclofenac-induced gastric ulcers and established its possible mechanisms of action.

Methods: In the in-vivo study, thirty six Sprague dawley rats of either sex weighing 150-200 grams were randomly assigned into a normal control (distilled water), negative control (distilled water plus diclofenac sodium), treatment (200 and 400 mg/kg Corchorus trilocularis Linn plus diclofenac sodium), positive control group (omeprazole plus diclofenac sodium), or comparison group (400 mg/kg aqueous leaf extract of spinach). The ulcer index, total acidity, volume, pH of gastric secretions, and gastric morphology were assessed. In the in-vitro anti-acidity study, a rat stomach was perfused with Kreb’s solution and the pH of the mucosal perfusate measured using a digital pH meter for 1 hour after exposure to histamine, acetylcholine, or pre-treatment with 400 mg/kg of the extract prior to addition the acid secretagogues.

Results: The extract, significantly reduced the ulcer index and total acidity in comparison to the diclofenac group. The high dose extract also increased the gastric pH and had cytoprotective effects. However, it did not significantly affect the volume of gastric secretions. In the in-vitro study, the extract significantly inhibited histamine and acetylcholine stimulated gastric acid secretions as analyzed by the areas under curve for pH against time.

Conclusions. The gastro-protective effects of Corchorus trilocularis Linn against diclofenac induced gastric ulcers are; therefore, mediated through preservation of the gastric mucosal barrier, increase in gastric pH, and inhibition of gastric acid secretion through the histamine H2 and acetylcholine M3 extracellular pathways on the parietal cell.

References

Patil AN, Advani MG, Mali SN, Pawar S, Raut SB. Evaluation of anti-ulcer effect of amlodipine in gastric ulcer models in rats. Indian J Pharmacol [Internet]. 2012 [cited 2019 Dec 3];44(3):387–9. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371465/

Malfertheiner P, Chan FKL, McColl KEL. Peptic ulcer disease. Lancet Lond Engl. 2009 Oct 24;374(9699):1449–61.

Nunes AP, Costa IM, Costa FA. Determinants of self-medication with NSAIDs in a Portuguese community pharmacy. Pharm Pract. 2016 Mar;14(1):648.

Nordin N, Salama SM, Golbabapour S, Hajrezaie M, Hassandarvish P, Kamalidehghan B, et al. Anti-Ulcerogenic Effect of Methanolic Extracts from Enicosanthellum pulchrum (King) Heusden against Ethanol-Induced Acute Gastric Lesion in Animal Models. PLoS ONE [Internet]. 2014 Nov 7 [cited 2019 Dec 3];9(11). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4224391/

Borrelli F, Izzo AA. The plant kingdom as a source of anti-ulcer remedies. Phytother Res PTR. 2000 Dec;14(8):581–91.

Khan M, Bano S, Javed K, Mueed MA. A comprehensive review on the chemistry and pharmacology of Corchorus species—A source of cardiac glycosides, triterpenoids, ionones, flavonoids, coumarins, steroids and some other compounds. In 2006.

Traditional-Medicinal-Flora-Habituated-In-Various-Regions-Of-Ysr-Kadapa-District-Andhra-Pradesh-India.Pdf [Internet]. [Cited 2019 Dec 3]. Available From: Https://Www.Researchgate.Net/Profile/Madhu_Reddy_Araveeti/Publication/293414697_Traditional_Medicinal_Flora_Habituated_In_Various_Regions_Of_Ysr_Kadapa_District_Andhra_Pradesh_India/Links/56b8421608ae44bb330bcd39/Traditional-Medicinal-Flora-Habituated-In-Various-Regions-Of-Ysr-Kadapa-District-Andhra-Pradesh-India.Pdf

PIssue166.pdf [Internet]. [cited 2019 Dec 3]. Available from: http://www.ajpls.com/admin/Issues/PIssue166.pdf

12.Ahirrao.pdf [Internet]. [cited 2019 Dec 3]. Available from: https://pharmacologyonline.silae.it/files/newsletter/2009/vol2/12.Ahirrao.pdf

Choudhary SB, Sharma HK, Karmakar PG, Kumar Aa, Saha AR, Hazra P, et al. Nutritional profile of cultivated and wild jute ('Corchorus’) species. Aust J Crop Sci [Internet]. 2013 Dec [cited 2019 Dec 3];7(13):1973. Available from: https://search.informit.com.au/documentSummary;dn=800810394284134;res=IELHSS

Al Batran R, Al-Bayaty F, Ameen Abdulla M, Jamil Al-Obaidi MM, Hajrezaei M, Hassandarvish P, et al. Gastroprotective effects of Corchorus olitorius leaf extract against ethanol-induced gastric mucosal hemorrhagic lesions in rats. J Gastroenterol Hepatol [Internet]. 2013 Aug [cited 2019 Jun 24];28(8):1321–9. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842111/

Mohammed Usman MR, Jhade D. In-Vitro Antacid Evaluation of Corchoru Depressus Linn. (harankhuri). Int J Pharm Life Sci. 2016 Sep;7(9):40–40.

Culling CFA, Allison RT, Barr WT. Cellular Pathology Technique. Elsevier; 2014. 651 p.

Bunce KT, Parsons ME, Rollings NA. The effect of metiamide on acid secretion stimulated by gastrin, acetylcholine and dibutyryl cyclic adenosine 3’,5’-monophosphate in the isolated whole stomach of the rat. Br J Pharmacol [Internet]. 1976 Sep [cited 2019 Dec 3];58(1):149–56. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1667127/

KoreKakasaheb J, Rajkumar S, PatelApsari J, KulkarniJitendra B. Antiulcer Activity Of Aqueous Extract Of Spinacia Oleracia In Rats. In 2011.

Peghini PL, Katz PO, Bracy NA, Castell DO. Nocturnal recovery of gastric acid secretion with twice-daily dosing of proton pump inhibitors. Am J Gastroenterol. 1998 May;93(5):763–7.

Phillipson M, Atuma C, Henriksnäs J, Holm L. The importance of mucus layers and bicarbonate transport in preservation of gastric juxtamucosal pH. Am J Physiol-Gastrointest Liver Physiol [Internet]. 2002 Feb 1 [cited 2019 Dec 3];282(2):G211–9. Available from: https://www.physiology.org/doi/full/10.1152/ajpgi.00223.2001

Wallmark B. Mechanism of Action of Omeprazole. Scand J Gastroenterol [Internet]. 1986 Jan 1 [cited 2019 Dec 3];21(sup118):11–6. Available from: https://doi.org/10.3109/00365528609090881

Prichard PJ, Yeomans ND, Mihaly GW, Jones DB, Buckle PJ, Smallwood RA, et al. Omeprazole: a study of its inhibition of gastric pH and oral pharmacokinetics after morning or evening dosage. Gastroenterology. 1985 Jan;88(1 Pt 1):64–9.

Tongen LA. The quantitative relationship between parietal cells and gastric acidity: Part I. Studies on the quantitative relationship between parietal cells and gastric acidity in human stomachs. Surgery [Internet]. 1950 Dec 1 [cited 2019 Dec 3];28(6):1009–15. Available from: https://www.surgjournal.com/article/0039-6060(50)90032-8/abstract

Schubert ML, Peura DA. Control of Gastric Acid Secretion in Health and Disease. Gastroenterology [Internet]. 2008 Jun 1 [cited 2019 Dec 3];134(7):1842–60. Available from: http://www.sciencedirect.com/science/article/pii/S0016508508007865

Kleveland PM, Waldum HL, Larsson H. Gastric Acid Secretion in the Totally Isolated, Vascularly Perfused Rat Stomach: A Selective Muscarinic-1 Agent Does, Whereas Gastrin Does Not, Augment Maximal Histamine-Stimulated Acid Secretion. Scand J Gastroenterol [Internet]. 1987 Jan 1 [cited 2019 Dec 3];22(6):705–13. Available from: https://doi.org/10.3109/00365528709011147

Schubert ML. Regulation of gastric acid secretion. Curr Opin Gastroenterol [Internet]. 1999 Nov [cited 2019 Dec 3];15(6):457. Available from: https://journals.lww.com/co-gastroenterology/Abstract/1999/11000/Regulation_of_gastric_acid_secretion.2.aspx

Yusuf S, Agunu A, Diana M. The effect of Aloe vera A. Berger (Liliaceae) on gastric acid secretion and acute gastric mucosal injury in rats. J Ethnopharmacol [Internet]. 2004 Jul 1 [cited 2019 Dec 3];93(1):33–7. Available from: http://www.sciencedirect.com/science/article/pii/S0378874104001199

Aihara T, Nakamura Y, Taketo MM, Matsui M, Okabe S. Cholinergically stimulated gastric acid secretion is mediated by M3 and M5 but not M1 muscarinic acetylcholine receptors in mice. Am J Physiol-Gastrointest Liver Physiol [Internet]. 2005 Jun 1 [cited 2019 Dec 3];288(6):G1199–207. Available from: https://www.physiology.org/doi/full/10.1152/ajpgi.00514.2004

Bunce KT, Parsons ME, Rollings NA. The effect of metiamide on acid secretion stimulated by gastrin, acetylcholine and dibutyryl cyclic adenosine 3’,5’-monophosphate in the isolated whole stomach of the rat. Br J Pharmacol [Internet]. 1976 Sep [cited 2019 Dec 3];58(1):149–56. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1667127/

Mesia-Vela S, Santos MT, Souccar C, Lima-Landman MTR, Lapa AJ. Solanum paniculatum L. (Jurubeba): Potent inhibitor of gastric acid secretion in mice. Phytomedicine [Internet]. 2002 Jan 1 [cited 2019 Dec 3];9(6):508–14. Available from: http://www.sciencedirect.com/science/article/pii/S094471130470149X


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