ORIGINAL ARTICLE

The antispasmodic effect of aqueous root bark extract of Carissa edulis (Forssk.) Vahl on isolated rabbit jejunum is mediated through blockade of calcium channels

LINCONE LINUS OLOUCH , BONIFACE MWANGI CHEGE, Cyril George siringo, Paul Mbugua Mungai, Anne Muriithi Muriithi Wangechi

LINCONE LINUS OLOUCH
UNIVERSITY OF NAIROBI. Email: bmchege87@gmail.com

BONIFACE MWANGI CHEGE
DEDAN KIMATHI UNVERSITY OF TECHNOLOGY

Cyril George siringo
university of nairobi

Paul Mbugua Mungai
university of Nairobi

Anne Muriithi Muriithi Wangechi
university of nairobi
Online First: April 25, 2020 | Cite this Article
OLOUCH, L., CHEGE, B., siringo, C., Mungai, P., Wangechi, A. 2020. The antispasmodic effect of aqueous root bark extract of Carissa edulis (Forssk.) Vahl on isolated rabbit jejunum is mediated through blockade of calcium channels. Discovery Phytomedicine 7(2). DOI:10.15562/phytomedicine.2020.124


Background

Spasms of the gut underlie hyperactive gut disorders. These conditions are highly prevalent and impart greater health care cost. Herbal antispasmodic remedies form a source of affordable, safe and easily available treatments in low resource areas. There is, therefore, a need to scientifically evaluate the therapeutic potential of these remedies. This study investigated the antispasmodic effect of aqueous root bark extract of Carissa edulis, herb used to manage hyperactive gut disorders such as abdominal colic and diarrhea.

Materials and methods

Pieces of jejunum were isolated from adult New Zealand White rabbits. They were mounted in an organ bath containing Tyrode’s solution. The rate and force of contraction were recorded using Powerlab coupled to Chart5 Software. The effects of the extract (0.1-10.0 mg/ml) on spontaneous jejunal contraction were investigated. The effect of 1.0 and 3.0 mg/ml extract was investigated on acetylcholine, KCl and CaCl2 induced contraction.

Results

Carissa edulis extract dose-dependently (0.1-10 mg/ml) significantly decreased the force but not the rate of spontaneous jejunal contraction. Extract (1 and 3 mg/) significantly decreased the magnitude of acetylcholine, KCl and CaCl2 induced contraction.

Conclusions

Aqueous root bark extracts of Carissa edulis possess a significant antispasmodic effect on rabbit jejunum. This appears to be through calcium channel blockade. These results validate its use as a remedy for hyperactive gut disorders.

 

References

Field M. Intestinal ion transport and the pathophysiology of diarrhea. J Clin Invest. 2003 Apr 1;111(7):931–43.

Walker CLF, Rudan I, Liu L, Nair H, Theodoratou E, Bhutta ZA, et al. Global burden of childhood pneumonia and diarrhoea. The Lancet. 2013 Apr 20;381(9875):1405–16.

Kaplan GG. The global burden of IBD: from 2015 to 2025. Nat Rev Gastroenterol Hepatol. 2015 Dec;12(12):720–7.

Annaházi A, Róka R, Rosztóczy A, Wittmann T. Role of antispasmodics in the treatment of irritable bowel syndrome. World J Gastroenterol WJG. 2014;20(20):6031.

Kigen G, Some F, Kibosia J. Ethnomedicinal plants traditionally used by the keiyo community in Elgeyo Marakwet County, Kenya. 2014;

Geissler PW, Harris SA, Prince RJ, Olsen A, Odhiambo RA, Oketch-Rabah H, et al. Medicinal plants used by Luo mothers and children in Bondo district, Kenya. J Ethnopharmacol. 2002 Nov 1;83(1):39–54.

Lincone OL, Mungai MP, Wangechi MA, George SC, Kayaja WD, Chege BM. Myorelaxant effect of aqueous root bark extract of Carissa edulis (Forssk.) Vahl. is mediated through activation of nitric oxide synthase. Afr J Pharm Pharmacol. 2019 Jun 8;13(10):124–33.

Grasa L, Rebollar E, Arruebo MP, Plaza MA, Murillo MD. The role of NO in the contractility of rabbit small intestine in vitro: effect of K+ channels. J Physiol Pharmacol Off J Pol Physiol Soc. 2005 Sep;56(3):407–19.

Cortes AR, Delgadillo AJ, Hurtado M, Domínguez-Ramírez AM, Medina JR, Aoki K. The antispasmodic activity of Buddleja scordioides and Buddleja perfoliata on isolated intestinal preparations. Biol Pharm Bull. 2006;29(6):1186–90.

Chokri A, Doukali R, El Abida K, Cheikh RB. Myorelaxant and spasmolytic effects of Globularia alypum L. extract on rabbit jejunum. Int J Pharmacol. 2010;6(5):608–15.

Kito Y, Mitsui R, Ward SM, Sanders KM. Characterization of slow waves generated by myenteric interstitial cells of Cajal of the rabbit small intestine. Am J Physiol-Gastrointest Liver Physiol. 2014;308(5):G378–88.

Devi RC, Sim SM, Ismail R. Spasmolytic effect of citral and extracts of Cymbopogon citratus on isolated rabbit ileum. J Smooth Muscle Res. 2011;47(5):143–56.

Alkizim FO, Matheka D, Abdulrahman FK, Muriithi A. Inhibitory effect of Mangifera indica on gastrointestinal motility. Med Chem Drug Discov. 2012;2(1):9–16.

Shah AJ, Gowani SA, Zuberi AJ, Ghayur MN, Gilani AH. Antidiarrhoeal and spasmolytic activities of the methanolic crude extract of Alstonia scholaris L. are mediated through calcium channel blockade. Phytother Res Int J Devoted Pharmacol Toxicol Eval Nat Prod Deriv. 2010;24(1):28–32.

Sanders KM, Koh SD, Ro S, Ward SM. Regulation of gastrointestinal motility—insights from smooth muscle biology. Nat Rev Gastroenterol Hepatol. 2012;9(11):633.

Gilani AH, Aziz N, Khan MA, Shaheen F, Jabeen Q, Siddiqui BS, et al. Ethnopharmacological evaluation of the anticonvulsant, sedative and antispasmodic activities of Lavandula stoechas L. J Ethnopharmacol. 2000;71(1–2):161–7.

Grasa L, Rebollar E, Arruebo MP, Plaza MA, Murillo MD. THE ROLE OF Ca2+ IN THE CONTRACTILITY OF RABBIT SMALL. J Physiol Pharmacol. 2004;55(3):639–50.


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