ORIGINAL ARTICLE

Evaluation of antioxidant, membrane stabilizing, antimicrobial, cytotoxic and thrombolytic properties of the crude extracts of Stixis suaveolens growing in Bangladesh

Md. Monirul Islam

Md. Monirul Islam
Professor,. Email: noory.chemistry@gmail.com
Online First: July 15, 2020 | Cite this Article
Islam, M. 2020. Evaluation of antioxidant, membrane stabilizing, antimicrobial, cytotoxic and thrombolytic properties of the crude extracts of Stixis suaveolens growing in Bangladesh. Discovery Phytomedicine 7(3). DOI:10.15562/phytomedicine.2020.125


The current study was conducted to investigate anti-oxidative, membrane stabilizing, antimicrobial, cytotoxic and thrombolytic properties of Methanol, Aqueous, Pet-Ether, Dichloromethane, and Ethyl Acetate soluble fractions of Stixis suaveolens. To determine the antioxidant activity, free radical scavenging activities (antioxidant capacity) of the plant extracts on the stable radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and total phenolic content was determined. Ascorbic Acid & BHT were used as standards in this studies. To evaluate cytotoxicity, the brine shrimp lethality bioassay was used.Vincristine sulfate (VS) was used as positive control. Antithrombolytic ability of the plant extracts were determined on bloods drawn from healthy volunteers. Membrane stabilizing potential were assessed by evaluating their ability to inhibit haemolysis of human erythrocytes. Antimicrobial investigation by disc diffusion method revealed no anti-microbial potential. The amount of total phenolic content differed in different extractives and ranged from 4.9375mg of GAE/gm of extractives to 70.125mg of GAE/gm of extractives of Fruits of S. suaveolens Roxb. The antioxidant activity of IC50 values in DPPH method are differed in different extractives and ranged from (75.67μg/ml) to (5634.00μg/ml). Methanol Soluble Fraction (MESFF) of Fruits of S. suaveolens Roxb. exhibited highest thrombolytic activity of 26.85%. Furthermore, the Aqueous Soluble Fraction (AQSF) inhibited 11.06%, Ethyl Acetate Soluble Fraction (EASF) inhibited 9.16%, Methanol Soluble Fraction (MESF) inhibited 8.84%, Dichloromethane Soluble Fraction (DCMSF) inhibited 7.92%, and Pet-Ether Soluble Fraction (PESF) inhibited 5.86% of hemolysis of RBC. The highest brine shrimp lethality was given by EASF with 0.99μg/ml followed by PESFwith 1.10μg/ml, DCMSFwith 4.77μg/ml, MESF with 8.37μg/ml and AQSFwith 24.26μg/ml. Hence, that S. suaveolens extracts and its fractions possess anti-oxidant, membrane stabilizing, cytotoxic and thrombolytic properties.

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