ORIGINAL ARTICLE

In vitro and in vivo Activity of Mist Amen Fevermix and Edhec Malacure, Polyherbal Antimalarial Products on Field Isolates of Plasmodium falciparum and Plasmodium berghei

Bernard Kofi Turkson

Bernard Kofi Turkson
University of Health and Allied Sciences, Institute of Traditional and Alternative Medicine (ITAM). Email: bentsi2000@yahoo.com
Online First: July 15, 2020 | Cite this Article
Turkson, B. 2020. In vitro and in vivo Activity of Mist Amen Fevermix and Edhec Malacure, Polyherbal Antimalarial Products on Field Isolates of Plasmodium falciparum and Plasmodium berghei. Discovery Phytomedicine 7(3). DOI:10.15562/phytomedicine.2020.129


Background

Malaria is a life-threatening infectious disease, which pose a public health challenge. Currently, reports of parasites resistance to artemisinin-based combination therapies is widespread. This has renewed calls for new antimalarial medications including  herbal products for the treatment of malaria. This study was undertaken to scientifically assess the antiplasmodial potentials of Mist Amen Fevermix and Edhec Malacure, two polyherbal antimalarial products used for the management of uncomplicated malaria, and establish their phytochemical constituents.

Methods

Qualitative phytochemical screening based on standard analytical methods. The antiplasmodial activity was assessed in vitro by using Plasmodium falciparum. The SYBR® Green assay was used to measure parasite growth inhibition. In vivo activity was assessed with Plasmodium berghei parasites using the Rane’s curative method with artesunate as positive control.

Results

The phytochemical screening of the products revealed the presence of alkaloids, flavonoids, tannin, steroid and saponin. In the in vitro studies, the IC50 values for Edhec Malacure was 70.89 ng/mL and 112.5ng/mL for Mist Amen Fevrmix. Artesunate exhibited an IC50 value of 1.571ng/mL. Edhec Malacure suppressed parasitemia by 76.17% (at 4.25mg/kg-1) and Mist Amen Fevermix by 69.03% (at 1.56 mg/kg-1) in vivo.

 

Conclusion

Mist Amen Fevrmix and Edhec Malacure Mixture demonstrated antiplasmodial activity and may be useful alternative antimalarial agents.

 

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