In vitro and in vivo Activity of Mist Amen Fevermix and Edhec Malacure, Polyherbal Antimalarial Products on Field Isolates of Plasmodium falciparum and Plasmodium berghei

Bernard Kofi Turkson

Bernard Kofi Turkson
University of Health and Allied Sciences, Institute of Traditional and Alternative Medicine (ITAM). Email: bentsi2000@yahoo.com
Online First: July 15, 2020 | Cite this Article
Turkson, B. 2020. In vitro and in vivo Activity of Mist Amen Fevermix and Edhec Malacure, Polyherbal Antimalarial Products on Field Isolates of Plasmodium falciparum and Plasmodium berghei. Discovery Phytomedicine 7(3). DOI:10.15562/phytomedicine.2020.129


Malaria is a life-threatening infectious disease, which pose a public health challenge. Currently, reports of parasites resistance to artemisinin-based combination therapies is widespread. This has renewed calls for new antimalarial medications including  herbal products for the treatment of malaria. This study was undertaken to scientifically assess the antiplasmodial potentials of Mist Amen Fevermix and Edhec Malacure, two polyherbal antimalarial products used for the management of uncomplicated malaria, and establish their phytochemical constituents.


Qualitative phytochemical screening based on standard analytical methods. The antiplasmodial activity was assessed in vitro by using Plasmodium falciparum. The SYBR® Green assay was used to measure parasite growth inhibition. In vivo activity was assessed with Plasmodium berghei parasites using the Rane’s curative method with artesunate as positive control.


The phytochemical screening of the products revealed the presence of alkaloids, flavonoids, tannin, steroid and saponin. In the in vitro studies, the IC50 values for Edhec Malacure was 70.89 ng/mL and 112.5ng/mL for Mist Amen Fevrmix. Artesunate exhibited an IC50 value of 1.571ng/mL. Edhec Malacure suppressed parasitemia by 76.17% (at 4.25mg/kg-1) and Mist Amen Fevermix by 69.03% (at 1.56 mg/kg-1) in vivo.



Mist Amen Fevrmix and Edhec Malacure Mixture demonstrated antiplasmodial activity and may be useful alternative antimalarial agents.



Dondorp A.M. Dondorp, François Nosten, Poravuth Yi, Debashish Das, Aung Phae Phyo, Joel Tarning, Khin Maung Lwin, Frederic Ariey, Warunee Hanpithakpong, Sue J. Lee, Pascal Ringwald, Kamolrat Silamut. Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009; 361: 455–467.DOI: 10.1056/NEJMoa0808859.

Evans, W. C. Trease and Evans' pharmacognosy. Elsevier Health Sciences. 2009.

Li, J.W.; Vederas, J.C. Drug discovery and natural products: End of an era or an endless frontier? Science. 2009; 325: 161–165. DOI: 10.1126/science.1168243.

Moll K, Ljungström. I, Perlmann H, Scherf. A, Wahlgren. M. Methods in Malaria Research. Manassas: University Boulevard. 2008.

Muregi F.W, Ishih. A, Miyase. T. Antimalarial Activity of Methanolic Extracts from Plants used in Kenyan Ethnomedicine and their Interactions with Chloroquine (CQ) against a CQ-Tolerant Rodent Parasite, in Mice. J Ethnopharmacol. 2007; 111: 190-5. DOI: 10.1016/j.jep.2006.11.009.

Newbold CJ, el Hassan SM, Wang J, Ortega ME, Wallace RJ. Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria. Br J Nutr. 1997; 78: 237-49. DOI: 10.1079/bjn19970143.

Phyo A.P, Ashley E.A, Anderson T.J.C, Bozdech Z, Carrara V.I, Sriprawat .K, Nair .S, White M.M, Dziekan .J, Ling C, Proux .S, Konghahong .K, Jeeyapant .A, Woodrow C.J, Imwong .M, McGready R., Lwin K.M, Day N.P.J, White NJ., Nosten F. Declining Efficacy of Artemisinin Combination Therapy Against P. Falciparum Malaria on the Thai-Myanmar Border (2003-2013): The Role of Parasite Genetic Factors. Clin Infect Dis. 2016; 63: 784-791. DOI: 10.1093/cid/ciw388.

R. Kaur and S. Arora. “Alkaloids-important therapeutic secondary metabolites of plant origin,” Journal of Critical Reviews. 2015 ; 2(3): 1–8.

Rasoanaivo P, Deharo E, Ratsimamanga-Urverg S, Frappier F. Guidelines for the non-clinical evaluation of the efficacy of traditional antimalarials In Traditional Medicinal Plants and Malaria. 2004; 256 –268.

Ryley J, Peters W. The Antimalarial Activity of Some Quinone Esters. Ann Trop Med Parasitol.1970; 84:20922. DOI: 10.1080/00034983.1970.11686683.

Shi, Q.W.; Li, L.G.; Huo, C.H.; Zhang, M.L.; Wang, Y.F. Study on natural medicinal chemistry and new drug development. Chin. Tradit. Herb. Drugs. 2010; 41: 1583–1589.

Soelberg. J, A. Asase, G. Akwetey, and A. K. Jäger. “Historical Versus Contemporary Medicinal Plant uses in Ghana,” Journal of Ethnopharmacology. 2015; l(160): 109–132. DOI:/10.1016/j.jep.2014.11.036.

Tarkang PA, Okalebo FA, Ayong LS, Agbor GA, Guantai AN. Anti-Malarial Activity of a Polyherbal Product (Nefang) during early and Established Plasmodium Infection in Rodent Models. Malar J. 2014; 13:456. DOI: 10.1186/1475-2875-13-456.

Toma A, Deyno S, Fikru A, Eyado A, Beale A. In Vivo Antiplasmodial and Toxicological Effect of Crude Ethanol Extract of Echinops kebericho Traditionally used in Treatment of Malaria in Ethiopia. Malar J. 2015; 14:196. DOI: 10.1186/s12936-015-0716-1.

Trager, W., and J. B. Jensen. Human Malaria Parasites in Continuous Culture. Science . 1976; 193:673-675. DOI: 10.1126/science.781840.

Van der Pluijm RW, Imwong M, Chau NH, Hoa NT, Thuy-Nhien NT, Thanh NV, Jittamala P, Hanboonkunupakarn B, Chutasmit K, Saelow C, Runjarern R, Kaewmok W, Tripura R, Peto TJ, Yok S, Suon S, Sreng S, Mao S, Oun S, Yen S, Amaratunga C, Lek D, Huy R, Dhorda M, Chotivanich K, Ashley EA, Mukaka M, Waithira N, Cheah PY, Maude RJ, Amato R, Pearson RD, Gonçalves S, Jacob CG, Hamilton WL, Fairhurst RM, Tarning J, Winterberg M, Kwiatkowski DP, Pukrittayakamee S, Hien TT, Day NP, Miotto O, White NJ, Dondorp AM. Determinants of dihydroartemisinin-piperaquine treatment failure in Plasmodium falciparum malaria in Cambodia, Thailand, and Vietnam: a prospective clinical, pharmacological, and genetic study. Lancet Infect Dis. 2019; 19: 952-961. DOI: 10.1016/S1473-3099(19)30391-3.

Waako P, Gumede B, Smith P, Folb P. The in Vitro and In Vivo Antimalarial Activity of Cardiospermum halicacabum L. and Momordica foetida Schumch Et. Thonn. J Ethnopharmacol. 2005; 99:137–43. DOI: 10.1016/j.jep.2005.02.017.

World Health Organization. Guidelines for the treatment of malaria. 3rd ed. Geneva. 2015.

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