Medical plants are used traditionally as alternative treatment for malaria. Anthocleista grandiflora (A. grandiflora) is used traditionally for the treatment of malaria.  This study attempt to scientifically validate its traditional use for malaria treatment by evaluating the antiplasmodial activity of its aqueous leaf extract (AAG) in Plasmodium berghei infected mice. P. berghei infected mice were orally treated with AAG (100, 200 and 400 mg/kg) daily in curative, suppressive and prophylactic studies. The untreated parasitized control (UPC) and the (PC) positive control were orally treated with normal saline (0.2mL) and chloroquine (CQ) (10mg/kg) respectively. At the end of treatment, blood samples were analyzed for parasitamia levels, hematological parameter and lipid profile. No morality was observed in acute toxicity evaluation of AAG in mice. AAG showed significant dose-dependent curative, suppressive and prophylactic activities via reductions in parasitamia levels at 100 mg/kg (p<0.05), 200 mg/kg (p<0.01) and 400 mg/kg (p<0.001) when compared to UPC. Mean survival time was increased in a dose-dependent fashion at 100 mg/kg (p<0.05), 200 mg/kg (p<0.01) and 400 mg/kg (p<0.001) when compared to UPC. AAG increased red blood cells, hemoglobin, pack cell volume, high density lipoprotein cholesterol levels, but decreased white blood cells, total cholesterol, triglyceride and low density lipoprotein cholesterol levels  in a dose-dependent fashion at100 mg/kg (p<0.05), 200 mg/kg (p<0.01) and 400 mg/kg (p<0.001) when compared to UPC. Comparatively the effects of AAG (400 mg/kg) were statistically at par with (CQ) 10mg/kg.  Based on the observations in this study, AGA seems safe and has antimalaria activity

Boon, R. (2010). Pooley's Trees of Eastern South Africa, A Complete Guide. Flora & Fauna Publications Trust, Durban.

Foden, W. & Potter, L. AnthocleistagrandifloraGilg. National Assessment: Red List of South African Plants version 2013.1. Accessed on 2014/02/05

Guyant, P., Corbel, V., Guérin, P.J. Past and new challenges for malaria control and elimination: the role of operational research for innovation in designing interventions. Malar J 2015 (14) 279; 1-11 doi: 10.1186/s12936-015-0802-4.

Harborne J B. Phytochemical Methods. Chapman and Hall Ltd., London; 1973; 49:180-188.

Iyamah AM, Idu MB Ethnomedicinal survey of plants used in the treatment of malaria in Southern Nigeria P.C. Jour of Ethnopharm 2015; 173; 287–302 doi: 10.1016/j.jep.2015.07.008

Knight, D.J. and Peters, W. The antimalarial action of N-Benzyl oxydihydrotriazines and the studies on its mode of action. Ann of Trop Med and Par. 1980; 74: 393-404. PMID: 7006531

Lorke, D. A new approach to practical acute toxicity testing. Arch. Toxicol. 1983; 54: 275-287. doi.org/10.1007/BF01234480

Magadulaa JJ, Mulhollanda DA and Croucha NR. Triterpenoids from Anthocleista grandiflora (Gentianaceae) Nat Prod Comm, 2008 (3) 6: 885-889

Muregi FW, Chlabra SC, Njagi ENM, Lang’at-Thoruur CC, Njue WM, Orago ASS, Omar SA, Ndiege IO. In vitro antiplasmodial activity of some plants used in Kissii, Kenya against malaria and their chloroquine potential effect. J. Ethnopharmacol. 2003;84: 235-239. doi: 10.1016/s0378-8741(02)00327-6

NottenA. (2014). Anthocleistagrandiflora. PlantZAfrica.com. retrieved from http://pza.sanbi.org/anthocleista-grandiflora

Ntie-Kang F, Amoa OP, Lifongo LL, Ndom JC, Sippl W, Mbaze LM. The potential of anti-malarial compounds derived from African medicinal plants, part II: a pharmacological evaluation of non-alkaloids and non-terpenoids. Malar J. 2014;13:81. doi: 10.1186/1475-2875-13-81

Odeghe, O. B.,Uwakwe, A. A., andMonago, C.C. Antiplasmodial Activity of Methanolic Stem Bark Extract of Anthocleistagrandiflora in Mice. Inter Jour of Appl Sci and Techn. 2012 (2) 4; 142-148.

Olanlokun JO, Babarinde CO and Olorunsogo O. O. Toxicity of Anchomanes difformis, An Antimalarial Herb in Murine Models Eur Jour of Med Plants 2017; 20(3): 1-13 doi.org/10.9734/EJMP/2017/35165

Ouédraogo M, Kangoye DT , Samadoulougou S , Rouamba T , Donnen P and Kirakoya-Samadoulougou F. Malaria Case Fatality Rate among Children under Five in Burkina Faso: An Assessment of the Spatiotemporal Trends Following the Implementation of Control Programs Int. J. Environ. Res. Public Health 2020, 17, 1840; 1-22 10.3390/ijerph17061840

Palmer, E. and Pitman, N. Trees of southern Africa. Volume 3. Cape Town: 1972; pp. 1845-1847.

Pasvol G. Management of severe malaria: interventions and controversies. Infect Dis Clin North Am. 2005;19:211–240. doi: 10.1016/j.idc.2004.10.007

Peters, W. Rational methods in the search for antimalarial drugs. Trans. R. Soc. Trop. Med. Hyg. 1967; 61: 400-410. doi.org/10.1016/0035-9203(67)90015-6

Petros Z. The need of standardized herbal remedies as alternate sources of antimalarial products in Ethiopia—updated review. Pharmacol Online. 2011;3:1440–7

Philip F. Uzor pages Alkaloids from Plants with Antimalarial Activity: A Review of Recent Studies Evidence-Based Compl and Altern Med 2020; 2020; 1-17 doi.org/10.1155/2020/8749083

Randrianarivelojosia, M., Rasidimanana, V.T., Rabarison, H. Plants traditionally prescribed to treat tazo (malaria) in the eastern region of Madagascar. Malar J 2003 (2) 25 ;1-9 doi: 10.1186/1475-2875-2-25

Ryley, J.F. and Peters, W. The antimalarial activity of some quinolone esters. Ann. Trop. Med. Parasitol. 1970; 84: 209-222.

Schmidt, Ernst; MervynLotter, Warren McCleland; scientific editor, John Burrows; illustrations, Sandie Burrows (2002). Trees and shrubs of Mpumalanga and Kruger National Park. Johannesburg: Jacana. p. 546.

Somsak V, Damkaew A, and Onrak P. Antimalarial Activity of Kaempferol and Its Combination with Chloroquine in Plasmodium berghei Infection in Mice. Jour of Path 2018; 2018;1- 7 doi.org/10.1155/2018/3912090

Sumbele NI, Sama OS , Kimbi, HK and Taiwe GS. Malaria, Moderate to Severe Anaemia, and Malarial Anaemia in Children at Presentation to Hospital in the Mount Cameroon Area: A Cross-Sectional Study Anemia. 2016; 2016, 1-12 doi: 10.1155/2016/5725634

Tangpukdee N, Duangdee C , Wilairatana P and Krudsood S. Malaria Diagnosis: A Brief ReviewKorean J Parasitol. 2009 47 (2) 93-102 doi: 10.3347/kjp.2009.47.2.93

Trease G and Evans W C. Pharmacognosy. Bailliere Tindall, London, Ed. 2008; 11, 45-50.

Wélé M, Keita S , Cisse C , Diarra N , Kirkman L and Baba-Moussa L Antibacterial and Antiplasmodial Activities of Tannins Extracted from Zizyphus mauritiana in Mali IJBCRR, 2018; 24(2): 1-8. DOI: 10.9734/IJBCRR/2018/45335

White, N.J. Anaemia and malaria. Malar J. 2018 (17) 371;1-17 doi: 10.1186/s12936-018-2509-9