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Aims: To provide knowledge on phytochemistry and bioactivity of H. madagascariensis Lam. ex Poiret.

Study Design: Multidisciplinary advanced bibliographic surveys, utilization of ChemBioDraw software package and dissemination of the resulted knowledge.

Place and Duration of Study: Faculty of Science, Pedagogical National University, Faculty of Science, University of Kinshasa and Department of Environmental Science, University of Gbadolite, the Democratic Republic of the Congo, between October 2018 and decembre 2019.

Methodology: A literature search was conducted to obtain information about the phytochemistry and pharmacognosy of H. madagascariensis from various electronic databases (PubMed, PubMed Central, Science Direct and Google scholar). The scientific name of this plant species was used as a keyword for the search, along with the terms phytochemistry and pharmacognosy. The chemical structures of the H. madagascariensis naturally occurring compounds were drawn using ChemBioDraw Ultra 15.0 software package.

Results : Harungana madagascariensis Lam. ex Poiret.  is a species of shrubs belonging to the family of Hypericaceae and is native to Madagascar and grows in the forests of tropical Africa, which is used in several traditional medicines to cure various diseases. The plant is reported to possess antibacterial, antifungal, antidiabetic, anti-inflammatory, hepatoprotective activity and vasodilatory effects. A wide range of chemical compounds including four prenylated anthranols, harunganols C−F, along with kenganthranol A, harunganin, and ferruginin A [were identified from the leaves and two anthronoids named harunmadagascarins A and B, along with the harungin anthrone, harunganol B, methyl 3-formyl-2,4-dihydroxy-6-methyl benzoate, friedelin, lupeol and betulinic acid were identified from the stem bark of H. madagascariensis.

Conclusion: This review can therefore help to inform future scientific research for the development of new drugs of relevance for improving human health and well-being. In particular, drug candidates for the treatment of diseases due to oxidative stress such as sickle cell disease.  


  1. Queensland Government. 2010. Harungana is a declared Class 3 pest plant under Queensland legislation. Primary industries and fisheries. Weeds, pest animals and ants. DEEDI Home. Queensland, Australia
  2. Burkill H.M., 1985. In: The useful Plants of West Tropical Africa (ed. 2) vol. 1-5, Royal Botanic Gardens, Kew, London.
  3. Burkill H.M., 1990. In: The useful Plants of West Tropical Africa (ed. 2) vol. 1-5, Royal Botanic Gardens, Kew, London.
  4. Orwa C.A., Mutua A., Kindt R., Jamnadass R., Anthony S., 2009. Agroforestree Database: a tree reference and selection guide version 4.0.
  5. Neuwinger H.D., 2000. African Traditional Medicine, a Dictionary of Plants Uses and Applications. 1st Edn. Medpharm Scientific Publishers, Stuttgart. ISBN: 978-3887630867.
  6. Bongo G.N., Ngbolua K.N., Masengo C.A., Karume K.L., Mukiza J., Tshilanda D.D., Tshibangu D.S.T., Ngombe N.K., Mbemba T.F., Mpiana P.T., 2017. Pharmacological screening of Gymnanthemum coloratum (Willd.) H. Rob. & B. Kahn (Compositae) and Terminalia ivorensis A. Chev. (Combretaceae) from DR Congo: Spotlight on the antisickling, antibacterial and antidiabetic activities. Tr. Plant Res 4(3): 441–448.
  7. Ngbolua K.N., Lengbiye E.M., Likolo J.B., Djolu R.D., Masengo C.A., Mpiana P.T., 2017. A mini-review on the pharmacognosy and phytochemistry of the tropical medicinal plant Annona senegalensis Persoon (Annonaceae). Trop Plant Res 4(1): 168-175.
  8. Ngbolua K.N., Rafatro H., Rakotoarimanana H., Ratsimamanga U.S., Mudogo V., Mpiana P.T., Tshibangu D.S.T., 2011a. Pharmacological screening of some traditionally-used antimalarial plants from the Democratic Republic of Congo compared to its ecological taxonomic equivalence in Madagascar. Int. J. Biol. Chem. Sci 5(5):1797-1804.
  9. Ngbolua K.N., Rakotoarimanana H., Rafatro H., Ratsimamanga U.S., Mudogo V., Mpiana P.T., Tshibangu D.S.T., 2011b. Comparative antimalarial and cytotoxic activities of two Vernonia species: V. amygdalina from the Democratic Republic of Congo and V. cinerea subsp vialis endemic to Madagascar. Int. J. Biol. Chem. Sci 5(1):345-353.
  10. Adeneye A.A., Olagunju J.A., Benebo A.S., Elias A.S., Adisa A.O., Idowu B.O., Oyedeji M.O., Isioye E.O., Braimoh O.B., Oladejo O.O., Alana E.O., 2008. Nephroprotective effects of the aqueous root extract of H. madagascariensis (L.) in acute and repeated dose acetaminophen renal injured rats. International Journal of Applied Research in Natural Products 1(1): 6 – 14.
  11. Adjanohoun E., 1981. Traditional medicine and African Plants. Organisation of African Unity/ Scientific Technical and research Commission (OAU/STRC).
  12. Agbor G.A., Kuate D., Oben J.E., 2007. Medicinal plants can be good source of anti-oxidants: case study in Cameroon. Pakistani Journal of Biological Sciences 10(4): 537 – 544.
  13. Irvine F.R., 1961. Woody plants of Ghana. London: Oxford University Press.
  14. Iwalewa E.O., Suleiman M.M., Mdee L.K., Eloff J.N., 2007. Antifungal and antibacterial activities of six different extracts of Harungana madagascariensis stem bark. Planta Medica 73(9): 890.
  15. Gill L.S., 1992. Ethnomedical Uses of Plants in Nigeria. Benin: Uniben Press, 130p.
  16. Olagunju J.A., Ogunfeibo A.B., Ogunbosi A.O., Taiwo O.A., 2004. Biochemical changes elicited by isosaline leaf and stembark extracts of Harungana madagascariensis in the rat. Phytotherapy Research 18: 588 – 591.
  17. Bouquet A., Debray M., 1974. Plantes médicinales de la Côte d’Ivoire. Travaux et Document de l’Orstom. 32 : 1-11. Edition de l’Office de la recherche Scientifique et technique Outre-mer
  18. Vasileva B., 1969. Plantes Médicinales de Guinee, Conakry, République de Guinée. Histoire Physique, University of Moscow, Russia
  19. Iwu M.M., 1993. In Handbook of African Medicinal Plants, CRC Press, Boca Raton, Fla.
  20. Hakizamungu E., Van Puyvelde L., Wery M., 1992. Screening of Rwandese medicinals plants of Anti-trichomonas activity. J. Ethnopharmacol, 36 (2): 143-146.
  21. Madubunyi I.I., Obi S.K.C., Nwebube N.I., Chime A.B., 1995. Antihepatotoxic and antimicrobial activities of H. madagascariensis leaf extracts. International Journal of Pharmacognosy, ,33(2), 129-134. (doi:10.3109/13880209509055212).
  22. Akah P.A., 1994. Abortifacient activity of some Nigerien medicinal plant. Phytotherapy Res. 8 (2), 106-108.
  23. Kokwaro J.O., 2009. Medicinal Plants of East Africa, 3rd Edition. In: Proceedings of 5th of African Crop Science Conference, Lagos, Nigeria. University of Nairobi Press.
  24. Gessler M.C., Nkunya M.H., Mwasumbi L.B., Heinrich M., Tanner M., 1994. Screening Tanzanian Medicinal Plants for Antimalarial Activity Acta Tropica, 56 (1): 65-77. doi: 10.1016/0001-706x(94)90041-8.
  25. Gessler M.C., Tanner M., Chollet J., Nkunya M.H.H., Heinrich M., 1995. Tanzanian medicinal plants used traditionally for the treatment of malaria: in vivo antimalarial and in vitro cytotoxic activities. Phytotherapy Research 9, N°7, 504–508.
  26. Abbiw D.K., 1990. In Useful Plants of Ghana, Intermediate Technology Publications and The Royal Botanic Gardens, Kew, London, S. 154-157.
  27. Maikere-Faniyo R., Van Puyvelde L., Mutwewingabo A., Habiyaremye F., 1989. J. Ethnopharmacol., 26, 101- 109. [PubMed].
  28. Sure Chem. 1998. Method of reducing blood glucose by administering Harunganin. Patent Number: 5837255. Issue Date: 1998-11-17.
  29. Adeneye A.A., Olagunju J.A., Elias S.O., Olatunbosun D.O., Mustafa A.O., Adeshile O.I., Ashaolu A.O., Laoye T.A., Bamigboye A.O., Adeoye A.O., 2008. Protective activities of the aqueous root extract of Harungana madagascariensis in acute and repeated acetaminophen hepatotoxic rats International Journal of Applied Research in Natural Products.,1(3), 29-42.
  30. Iwalewa E.O., Naidoo V., Bagla V., Eloff J.N., 2009. Nitric oxide producing effect of six extracts from Harungana madagascariensis Lam. ex Poiret (Guttiferae) stem bark. African Journal of Pharmacy and Pharmacology, 3(1), 014-021. Available online at: ISSN 1996-0816.
  31. Iwalewa E.O., Adewale I.O., Taiwo B.J., Arogundade T., Osinowo A., Daniyan O.M., Adetogun G.E., 2008. Effects of Harungana madagascariensis Stem Bark Extract on the Antioxidant Markers in Alloxan Induced Diabetic and Carrageenan Induced Inflammatory Disorders in Rats. Journal of Complementary and Integrative Medicine, 5(1), 1553-3840. DOI: 10.2202/1553-3840.1088.
  32. Dalziel J.M., 1959. The Useful Plants of West Tropical Africa. London: Crown Agents for Overseas Government and Administration, 424 p.
  33. Iwalewa E.O., Suleiman M.M., Mdee L.K., Eloff J.N., 2009. Antifungal and antibacterial activities of different extracts of H madagascariensis stem bark. Pharm Biol; 47:878–885.
  34. Munro A., Bassir O., 1969. Oxalate in Nigeria vegetables. WAJ Biol Appl. Chem; 12:14–17.
  35. Blood D.C., Radostits O.M., 1989. Veterinary Medicine. 7th edition, Balliere Tindall, London, p. 589.
  36. Banso A., Adeyemo S.O., 2007. Evaluation of antimicrobial properties of tannins isolated from Dichrostachys cinerea. Afr J Biotechnol; 6:1785–1787.
  37. Claude B., Paule S., 1979. The manual of natural living. 1st edition. Biddles Ltd., London, 98–101.
  38. Aremu C.Y., Udoessien E.I., 1989. Chemical estimation of some inorganic elements in selected fruits and vegetables. Food Chem: 37:227–234.
  39. Gbolahan D., 2001. Lesson Note on Medical Importance of Trace Elements. Centre for Natural Health Studies, Surulere, Nigeria, p. 67.
  40. Lee H., Jung E.Y., Suh H.J., 2009. Chemical composition and anti-stress effects of yeast hydrolysate. J Med Food: 12:1281–1285.
  41. Bursal E., Koksal E., Gulcin I., Goren A.C., 2013. Antioxidant activity and polyphenol content of cherry stem (Ceraus avium L) determined by LC-MS/MS. Food Res Int : 51:66–74.
  42. Bassey S.A., Basil N.I., Uwemedimo E.U., 2015. Nutrient Composition and in vitro Antioxidant Properties of H. madagascariensis Stembark Extracts. Journal of medicinal Food. J Med Food 18 (5) 609–614. Mary Ann Liebert, Inc., and Korean Society of Food Science and Nutrition. DOI: 10.1089/jmf.2014.0084.
  43. Stray F., 1998. The Natural Guide to Medicinal Herbs and Plants. Tiger Books International, London, pp. 12–16.
  44. Mayasuki Y., Stoichi H., 1997. Extraction of blood glucose sugar lowering saponins from Beta vulgaris. Chem Abstr: 20:14–17.
  45. Price K.R., Johnson I.T., Frederick G.R., 1997. The chemical and biological significance on saponins in food in feeding stuff. CRC Crit Rev Food Sci Nutr: 5:56–59.
  46. David H., 1983. The New Holistic Herbal. 3rd Edition. Findhorn press, USA, p. 241.
  47. Akpan M.M., Odeomena C.S., Nwachukwu C.N., Danladi B., 2012. Antimicrobial assessment of ethanolic extract of Costus afer leaves. Asian J Plant Sci Res: 2: 335–341.
  48. Oluwatosin O., Johnson, Ming Zhao, Gunn J., Bernard D., Santarsiero, Zhi-Qi Yin, Gloria A., Ayoola, Coker H.A.B., Chun-Tao., 2014. Che.α-Glucosidase Inhibitory Prenylated Anthranols from Harungana madagascariensis, Journal of Natural Products, DOI: 10.1021/acs.jnatprod.5b00924
  49. Kouam S.F., Ngadjui B.T., Karsten Krohn., Wafo P., Asma Ajaz, Choudhary M. I., 2005. Prenylated anthronoid antioxidants from the stem bark of Harungana madagascariensis. Phytochemistry 66: 1174–1179.
  50. Ebelle Etame R.M., Mouokeu R.S., Ngono Ngane R.A., Assam Assam J.P., Masoohe A. M., Tientcheu R.0., Hopogap M.L., Etoa F.X., 2017. Acute and sub-acute toxicity of Harungana madagascariensis LAM (Hypericaceae) stem bark methanol extract. Journal of Applied Pharmaceutical Science Vol. 7 (03), pp. 160-167.
  51. Nimenibo-Uadia R., Nwachukwu K., 2017. Biochemical evaluations of H. madagascariensis lam aqueous leaf extract in diabetic rats. International Research Journal of Natural Sciences 5(2): 1-11.
  52. Mathew B.A., Yusuf M.N., Abu S.J., Fakolade B.A., Ibrahim A., 2016. An Inhibitory Property of Crude Ethanolic Extract of H. Madagascariensis Root on New Castle. International Journal of Engineering and Applied Sciences 3(12): 26-29. ISSN: 2394-3661
  53. Inman W., DeWald, Luo J., 1998. Method of reducing blood glucose by administering Harunganin or Vismin. United State Patent [19] number US005837255A.
  54. Okoli A.S., Okeke M.I., Iroegbu C.U., Ebo P.U., 2002. Short communication. Antibacterial Activity of H. madagascariensis Leaf Extracts. Phytotherapy Research. Phytother. Res. 16: 174–179.
  55. Ngo E.L. T., Billong M.J.R., Nyemb N., Fouda B.Y., Longo F., Kouam F.S., Dimo T., 2018. Vasodilatory Effects of Aqueous Extract from H. madagascariensis Stem Bark in Isolated Rat Aorta: The Roles of Endothelium and K+ Channels. American Journal of Ethnomedicine. 5(1): 8. DOI: 10.21767/2348-9502.10008.
  56. Adebayo A., Gbolade., Tira-Picos V., Nogueira J.M.F., Adekunle T., Oladele., 2009. Comparative analysis of essential oils from the leaf, fruit and stem bark of Harungana madagascariensis. International Journal of Green Pharmacy, 290-293. DOI: 10.4103/0973-8258.59734
  57. Medewase, J.O., Ezike A.C., 2018. Preliminary investigation of the anti-inflammatory activity of H. madagascariensis leaf extract Lam. Expoir (Hypericaceae). Full Length Research Paper. Article Number: 0D49695. A Paper Presented at the Wannpres 9th Sub-Regional Scientific. Meeting, University of Nigeria, Nsukka, Enugu State, Nigeria. 30th May – 2nd
  58. Moulari B., Lboutounne H., Pellequer Y., Guillaume Y.C., Millet J., Pirot F., 2005. Vectorization of H. madagascariensis Lam. Ex Poir. (Hypericaceae) Ethanolic Leaf Extract by Using PLG-Nanoparticles: Antibacterial Activity Assessment. Drug Development Research 65: 26–33
  59. Toty A.A., Guessennd N., Bahi C., Kra A.M., Otokore D.A., Dosso M., 2013. Évaluation in-vitro de l’activité antibactérienne de l’extrait aqueux de l’écorce de tronc de Harungana madagascariensis sur la croissance de souches multi-résistantes. Bulletin de la Société Royale des Sciences de Liège, 82 : 12 – 21
  60. Iwalewa E.O., Omisore, N.O., Adewunmi C.O., Gbolade A.A., Ademowo O.G., Nneji, C., Agboola O.I., Daniyan O.M., 2008. Anti-protozoan activities of Harungana madagascariensis stem bark extract on trichomonads and malaria. J. Ethnopharmacol. 117: 507–511.
  61. Mouthé H.G., Laure M.T.G., Gbetnkoma B.Y.M.., Hussaind H., Greene I.R., Tchaleu N.B., Kouama S.F., 2020. Phytochemistry and pharmacology of Harungana madagascariensis: mini review. Phytochemistry Letters 35 (2020) 103–112
  62. Biapa P.C.N., Matei H., Bâlici S., Oben J.E., Ngogang J.Y., 2013. Protective effects of stem bark of Harungana madgascariensis on the red blood cell membrane. BMC Compl. Altern. Med. 13: 98.
  63. Mpiana P.T., Makelele L.K., Oleko R.W., Bokota M.T., Tshibangu D.S.T., Ngbolua K.N., Mbala M.B., Atibu E.K., Nshimba S.M., 2010. Antisickling activity of medicinal plants used in the management of Sickle cell Disease in Tshopo district, D.R.Congo, Australian Journal of Medical Herbalism 22 (4): 132-137.

How to Cite

Ngbolua, K.- te-N. (2020). Review on ethnobotany, phytochemitry and bioactivity of the Tropical medicinal plant species Harungana madagascariensis Lam. ex Poiret. (Hypericaceae). Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 7(3).




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