ORIGINAL ARTICLE

Phytochemical profile, antioxidant and anthelmintic activities of O. gratissimum leaves collected in Kinshasa (D.R. Congo)

Pius Tshimankinda Mpiana

Pius Tshimankinda Mpiana
UNIVERSITE DE KINSHASA, FACULTE DE SCIENCE B.P. 190 KINSHASA 11 D R CONGO. Email: ptmpiana@gmail.com
Online First: October 15, 2020 | Cite this Article
Mpiana, P. 2020. Phytochemical profile, antioxidant and anthelmintic activities of O. gratissimum leaves collected in Kinshasa (D.R. Congo). Discovery Phytomedicine 7(4). DOI:10.15562/phytomedicine.2020.145


Extracts of natural plants contain several chemical groups with various biological activities. This work aimed to evaluate the antioxidant, anthelminthic activities and determining the secondary metabolites, mineral and histological elements of Ocimum gratissimum from Democratic Republic of the Congo used for various culinary and therapeutic purposes. Primo Star 200® microscope was used for microscopic analysis further thin layer chromatography and X-ray fluorescence were used for chemical analysis. Antioxidant and anthelminthic activities were evaluated with DPPH° radical scavenging method and the rate of Benhamia rosea death, respectively. Spiral vessels, group scleritis, sclerotic fiber, pollen seed, stomata, hairs cells were found by Powder microscopic analysis. Secondary metabolites such as polyphenols (tannins, flavonoids, anthocyanins, leuco anthocyanins), alkaloids, steroids, terpenoids and various mineral elements (K, Ca, Cl, P, S, Mg, Na, Zn, Cu, Mn, Fe, etc.) were found in O. gratissimum leaves. Aqueous extract showed a high antioxidant activity with an IC50 of 25.7 ± 1.03 μg/mL. Aqueous extract of O. gratissimum leaves is a good antioxidant and it has an interesting anthelmintic activity.

References

Mbadiko CM, Inkoto1 CL, Gbolo1 BZ, Lengbiye EM, Kilembe JT, Matondo A, Mwanangombo DT, Ngoyi EM et al. A Mini Review on the Phytochemistry, Toxicology and Antiviral Activity of Some Medically Interesting Zingiberaceae Species. Journal of Complementary and Alternative Medical Research. 2020; 9(4): 44-56

TshilandaDD, OnyambokoDV, MwanangomboDT, TsaluPV, MisengabuNK, TshibanguDST, NgboluaKN, Mpiana PT. In vitro Antisickling Activity of Anthocyanins from Ocimum canun (Lamiacea). Journal of Advancement in Medical and Life Sciences. 2015;3. DOI: 10.13140/RG.2.1.3176.5609. (a)

Ngbolua KN, Moke EL, Mbembo MB, BongoGN, Kapepula PM, Ngombe NK, Messi M, Mbing JN, Pegnyemb DE, Mpiana PT. Selenium Content, Anthelmintic, Antioxidant and Antibacterial Activities of Artocarpus Heterophyllus Lam. From Ubangi Ecoregion in Democratic Republic of the CongO. Am J Biomed Sci & Res. 2019; 6(2)DOÏ : 10.34297/AJBSR.2019.06.001013.

Qureshi SM, Patel J, Giri CI, Hasan S, Shaik M, et al. In vitro anthelmintic activity of root extract of Trapabispiniosa Roxb. Against Pheretimaposthuma and Ascardiagilli. Study of anthelmintic activity. Int J of Pharma Sc and Research. 2010; 1(9): 353-356.

Ba K, Tine E, Destain J, Cissé N, Thonart P. Comparative study of phenolic compounds, antioxidant capacity of different varieties of Senegalese sorghum and the amyllytic enzymes of their malt. Biotechnol. Agron. Soc. Environ. 2010; 14(1): 131-139.

Bouzid W, Yahia M, Abdeddaim M, Aberkane MC, Ayachi A. Evaluation of the antioxidant and antimicrobial activities of monogynous hawthorn extracts.LebaneseScienceJournal. 2011; 12(1): 59-69.

KapepulaPM, MungitshiPM, FranckT, MickaladAM, NgoyiDM, KalendaPDT, NgombeNK, SerteynD, TitsM, FrédérichM, MuyembeJJT. Antioxidant potentiality of three herbal teas consumed in Bandundu rural areas of CongO. Nat Prod Res.2017; 31(16): 1940-1943.

InkotoCL, BongoGN, MutwalePK, MasengoAC, GboloZB, TshiamaC, NgombeBK, ItekuJB, MbembaTF, MpianaPT, NgboluaKN. Microscopic features and chromatographic fingerprints of selected congolese medicinal plants: Aframomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Emergent Life Sciences Research.2018;4(1): 1-10.

Bruneton J. Pharmacognosy, Phytochemistry of Medicinal Plants. 3rd Edition, Revue et Augmentée, Tec & Doc,Paris, 1999.

Wagner H, Bladt S, Zgainski EM. Plant Drug Analysis ; A Thin Layer Chromatography Atlas, 1984, Allemagne.

Kitadi JM, Inkoto CL, Lengbiye EM, Tshibangu DST, Tshilanda DD, Ngbolua KN, Taba KM, Mbala BM, Schmitz B, Mpiana PT. Antisickling activity and mineral content of Hura crepitans L., Alternanthera bettzichiana (regle) G. Nicholson and Dissotis brazzae cogn, plants used in the management of sickle cell disease in Kwilu province, Democratic Republic of the Congo. European Journal of Pharmaceutical and Medical Research. 2019; 6(12): 79-83

Bongo G, Inkoto C, Masengo C, Tshiama C, Lengbiye E, Djolu R, Mutwale K, Ngombe K, Mbemba T, Tshilanda D, Mpiana P, Ngbolua KN. Assessment of Antisickling, Antioxidant and Antibacterial Activities of Some Congolese Taxa: Aframomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia Whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine. 2017; 2(4): 52-59. Doi: 10.11648/j.ajlm.20170204.13.

Yashaswini T, Akshara KAM, Prasad K, Kumar MD, Akshaykumar K. Anthelmintic activity of seed extracts of artocarpus heterophyllus.IOSR Journal ofnpharmacy and biological sciences. 2016; 11(5): 19-23.

Tshilanda DD. Etude phytochimique et activité anti-drepanocytaire de trois espèces du genre Ocimum: Ocimum basilicum l., O. canum sims, O. gratissimum l. (Lamiaceae). Thèse de doctorat.2016

Tshilanda DD, Onyamboko DNV, Babady PB, Ngbolua KN, Tshibangu DST, Dibwe EF, Mpiana PT. Anti-sickling Activity of Ursolic Acid Isolated from the Leaves of O. gratissimum L. (Lamiaceae). Nat. Prod. Bioprospect. 2015; 5: 215-221. DOI 10.1007/s13659-015-0070-6 (b)

Mpiana PT, Ngbolua KN, Bokota MT, Kasonga TK, Atibu E.K & Mudogo V. In vitro Effetcts of Anthocyanins Extracts From Justicia secunda VAHL on the Solubility of Hemoglobin S and Membrane Stability of Sickle Erythrocytes, Blood Transfusion. 2010. DOI: 10.250/2009.0120-09.

Pachkore GL and Dhale DA. Phytochemicals, vitamins and minerals content of three ocimum species. International Journal of Science Innovations and Discoveries. 2012;2(1): 201-207.

Alvarez SI and Marcén JFE. General characteristics of trace elements: Diagnostic algorithms and methods. In clinical laboratory trace elements: Action on health and its role in the pathologies. 2012 1-36.

Martinez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz LC, Bastias E et al. Carvajal Minerals in plant food: effect of agricultural practices and role in human health. A review Agron. Substain. Dev. 2010; 30: 295-309. DOI: 10.1051/agro/2009022.

Kouassi EK, Ouattara S, Seguin C, Fournel S, Frisch B. Study of some biological properties of O. gratissimum L., a Lamiaceae harvested in Daloa (Ivory Coast) European Scientific Journal. 2018; 14(3): 477-493.

KapepulaPM, KatumbayDT, MumbaD, FrédérichM, MbembaT, NgombeNK. Traditional foods as putative sources of antioxidants with health benefits in Konzo, antioxidants in foods and its applications. Emad shalaby and ghada mostafa azzam.intechopen. DOI : 10.5772/intechopen.74523.

AkinmoladunCA, IbukunEO, AforE, ObuotorEM, Farombi EO.Phytochemical constituent and antioxidant activity of extract from the leaves of O. gratissimum. Scientific Research and Essay. 2007; 2(5): 163-166.

TshilandaDD, InkotoCL, Mpongu K, MataS, MutwalePK, TshibanguDST, BongoGN, NgboluaKN, MpianaPT. Microscopic Studies, Phytochemical and Biological Screenings of Ocimum canum. International Journal of Pharmacy and Chemistry. 2019; 5(5): 61-67. Doi: 10.11648/j.ijpc.20190505.13.

Alawa CBI, Adamu AM, Gefu JO, Ajanusi OJ, Abdu PA, Chiezey NP et al. In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity. Veterinary Parasitology. 2003; 113(1): 73-81

Kumarasingha R, Preston S, Yeo T-C, Lim DSL, Tu C-L, Palombo EA et al. Anthelmintic activity of selected ethno-medicinal plant extracts on parasitic stages of Haemonchus contortus.Parasites & Vectors. 2016; 187(9): 1-7. DOI 10.1186/s13071-016-1458-9.


No Supplementary Material available for this article.
Article Views      : 0
PDF Downloads : 0