Ethanol and organic acid extracts from the leaves of Vitex madiensis Oliv. were evaluated for their antihelminthic activity using the animal model of Benhamia rosea and B. itoleisis (Kinshasa/DRC earthworms), antibacterial activity and antioxidant activity. Different concentrations (5 mg/mL, 2.5 mg/mL, 1.25 mg/mL and 0.625 mg/mL) of standard Albendazole (positive control) and sample solutions (organic acid extracts and ethanol extracts) were used for this study which involved the determination of the paralysis time (vermifuge) and mortality time (vermicidal activity) of the worms. The presence of different secondary metabolites was determined by the thin layer chromatography. The results obtained showed that the ethanolic extract and the organic acid extracts showed significant antihelminthic activity. Microscopic examination of the parts used revealed the presence of various histological elements. Its phytochemical profile remains dominated by various secondary metabolites such as coumarins, anthraquinones, flavonoids, phenolic acids and terpenoids. The mineral composition was determined by inductively coupled argon plasma optical emission spectrometry (ICP-EOS) showed the abundance of various elements, namely calcium, iron, potassium, selenium, sodium, magnesium, manganese, nickel, phosphorus, cobalt, copper, barium and zinc in this plant. The antibacterial activity of the extracts of this plant was weak against the bacterial strains tested. The organic acid and ethanol extracts showed interesting antioxidant activity. The leaves extracts of Vitex madiensis Oliv. showed good antihelminthic, antibacterial and antioxidant activities related to their phytochemical compositions. All these results constitute scientific evidence validating the use of this medicinal plant for the management of parasitic infections of small ruminants in the Democratic Republic of the Congo.
Ajay KM, Niranjan US, Rao MM, Padhi MM, Ramesh B. A review of the important chemical constituents and medicinal uses of Vitex genus. Asian Journal of Traditional Medicines. 2011 ; 6(2):54-59.
Chantaranothai P. A revision of the genus Vitex (Lamiaceae) in Thailand. Trop. Nat. Hist. 2011 ; 11:91-118.
Ngbolua KN, Bishola TT, Mpiana PT, Mudogo V, Tshibangu DST, Ngombe KN, et al. In vitro antisickling and free radical scavenging activities of Pentaclethra macrophylla Benth. (Fabaceae). Journal of Advancement in Medical and Life Sciences. 2014 ; 1(2). DOI: 10.15297/JALS.V1I2.03.
Latham P. Useful plants of Bas-Congo province, Democratic Republic of the Congo. DFIG, London, United Kingdom. 2004 ; 320p.
Padmalatha K, Jayaram K, Raju NL, Prasad MNV, Arora R. Ethnopharmacological and biotechnological significance of Vitex. Biorem. Biod. Bioavailaility. 2009 ; 3:6-14.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical Screening and Extraction: A review. Int Pharmac Scienc. 2011 ; 1(1): 98-106.
Kapepula PM, Mungitshi PM, Franck T, Mouithys Mickalad A, Ngoyi DM, et al. Antioxidant potentiality of three herbal teas consumed in Bandundu rural areas of Congo. Nat Prod Res. 2016 ; 31(16): 1940-1943.
Yashaswini T, Akshara KAM, Prasad K, Kumar MD, Akshaykumar K. Anthelmintic activity of seed extracts of Artocarpus heterophyllus. IOSR Journal of Pharmacy and Biological Sciences. 2016 ; 11(5): 19-23.
Ngombe NK, Ngolo CN, Kialengila DM, Wamba AL, Mungisthi PM, Tshibangu PT, Dibungi PKT, Kantola PT, Kapepula PM. Cellular Antioxidant Activity and Peroxidase Inhibition of Infusions from Different Aerial Parts of Cassia occidentalis. Journal of Biosciences and Medecine. 2019 ; 7, 83â€“94.
Inkoto LC, Bongo NG, Kapepula MP, Masengo AC, Gbolo ZB, Tshiama C, Ngombe KB, Iteku BJ, Mbemba FT, Mpiana PT, Ngbolua KN. Microscopic features and chromatographic fingerprints of selected congolese medicinal plants: Aframomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. Emergent Life Sciences Research. 2018 ; 4 (1): 1-10.
Bongo G, Inkoto C, Masengo C, Tshiama C, Lengbiye E, Djolu R. and Kapepula M et al. Antisickling, Antioxidant and Antibacterial Activities of Afromomum alboviolaceum (Ridley) K. Schum, Annona senegalensis Pers. and Mondia whitei (Hook. f.) Skeels. American Journal of Laboratory Medicine. 2017 ; 2: 52-59.
Dash GK, Suresh P, Sahu SK, Kar DM, Ganapaty S, and Panda SB. Evaluation of Evolutus alsinoides Linn. For antihelminthic and antimicrobial activities. Journal of Natural Remedies. 2002 ; (2): 182-185.
Doaigey AR, El-Zaidy M, Alfarhan A, Milagy AES, Jacob T. Pollen morphology of certain species of the family Lamiaceae in Saudi Arabia. Saudi Journal of Biological Sciences. 2018 ; 25 (2): 354â€“60. https://doi.org/10.1016/j.sjbs.2017.03.001.
Sivagnanasundaram, Karunanayake KOLC. Phytochemical Screening and Antimicrobial Activity of Artocarpus heterophyllus and Artocarpus altilis Leaf and Stem Bark Extracts. OUSL Journal. 2015 ; (9): 1- 17.
Soetan KO, Olaiya CO and Oyewole OE. The importance of mineral elements for humans, domestic animals and plants: A review. African Journal of Food Science. 2010 ; 4(5) pp. 200-222.
Arinola OG, Nwozo SO, Ajiboye JA, Oniye AH. Evaluation of trace elements and total antioxidant status in Nigerian cassava processors Pak, Nutr. 2008 ; 7(6): 770-772.
Quintin A and Frank J. Veterinary anthelmintics: old and new. Trends in Parasitology. 2004 ; 23(20): 17-25.
Panda SK, Das D and Tripathy NK. Evaluation of anthelmintic activity of Chlorophytum borivilianum santapau & fernandes. Int J Res Pharm Biomed. 2011 ; Sci; 21: 571-576.
Coles GC, Jackson F, Pomroy WE, Prichard RK, Von samson-Himmelstjerna G, et al. The detection of anthelmintic resistance in nematodes of veterinary importance. Vet. Parasitol. 2006 ; 136(3-4): 167-185.
Ngbolua KN, Mubindukila NR, Mpiana PT, Tshibangu DST, Masengo CA, et al. Phytochemical screening, Antibacterial and Antioxidant activities of Anthocleista liebrechtsiana Wild & T Durand (Gentianaceae) originated from Democratic Republic of the Congo. Journal of Advancement in Medical and Life Sciences. 2014 ; 1(3): 13.
Ngbolua KN, Mubindukila NR, Mpiana PT, Masengo CA, Baholy R, et al. In vitro Assessment of Antibacterial and Antioxidant activities of a Congolese medicinal plant species Anthocleista schweinfurthii Gilg (Gentianaceae). J of Modern Drug Discovery and Drug Delivery Research. 2014; pp. 113.
Min BR, Barry TN, Attwood GT, Mc Nabb WC. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Anim. Feed Sci. Technol. 2003 ; 106(1-4): 3-19.
Molan AL, Sivakumaran S, Spencer PA and Meagher LP. Green tea flavan-3-ols and oligomeric proanthocyanidins inhibit the motility of infective larvae of Teladorsagia circumcincta and Trichostrongylus colubriformis in vitro. 2004 ; Res. Vet. Sci. 77(3): 239â€“243.
How to Cite
Ngbolua, K.- te-N. (2020). Microscopic Studies, Mineral composition and Bioactivity of Vitex madiensis Oliv. (Lamiaceae). Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 7(4). https://doi.org/10.15562/phytomedicine.2020.149