ORIGINAL ARTICLE

Phytochemical, antioxidant and antibacterial activities of the aqueous and ethanol extracts of Pinus halepensis

bouzenna hafsia , Samout noura, guesmi fatma, dhibi sabah, issam saidi, Smida Arij, khdher Ahd, akermi Sarra, elfkih Abdelfateh, Hfaiedh Najla

bouzenna hafsia
. Email: bouzenna.hafsia@hotmail.fr

Samout noura


guesmi fatma


dhibi sabah


issam saidi


Smida Arij


khdher Ahd


akermi Sarra


elfkih Abdelfateh


Hfaiedh Najla

Online First: January 15, 2021 | Cite this Article
hafsia, b., noura, S., fatma, g., sabah, d., saidi, i., Arij, S., Ahd, k., Sarra, a., Abdelfateh, e., Najla, H. 2021. Phytochemical, antioxidant and antibacterial activities of the aqueous and ethanol extracts of Pinus halepensis. Discovery Phytomedicine 8(1). DOI:10.15562/phytomedicine.2021.157


The present study was designed to evaluate the phytochemical screening of different extracts (Aqueous and ethanol) of the needles of Pinus halepensis; to explore their antioxidant activities with the different tests and to determine their antibacterial proprieties. Our results revealed that these extracts contain polyphenols, flavonoids and tannins. Then, the same extracts(Aqueous and ethanol)present the highest content of total phenol and tannins which were of order respectively(735.41 ± 0.09 µgGAE/ mg of extract and 49.60 ± 0.012 µg CE /mg of extract compared with other extracts. The results showed alsothat the different extracts had an important antioxidant andantibacterial activity. Overall, the richness of the different extract of Pinus halepensis with total polyphenols, flavonoids and tannins explained the biologic propriety of this plant.

Keywords: Antibacterial activity, Antioxidant activity,Pinus halepensis, phytochemical study.

References

Dob, R.I., Szachowicz-Petelska, B., Skrzydlewska,E.,Figaszewski, Z. (2008).Effects of green tea on physico-chemical properties of liver cell membranes of rats intoxicated with ethanol. Polish J. Environ. 17 (3), 327–333.

Sadou, N., Seridi, R., Djahoudi, A., Youcef, H. (2015). Chemical composition and antibacterial activity of the needls essential oil of Pinushalepensis Mill.from north east of Algeria. Rev. Sci. Technol. Synthèse.30, 33-39.

Ghedira, K. (2005). Les flavonoïdes: structure, propriétés biologiques, rôle prophylactique et emplois en thérapeutique. PhytothérapieIEC-6 cells. Archives of Physiology and Biochemistry. 364-370.

Kabera, JN., Semana E., Mussa AR., He X. (2014).Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. Journal of Pharmacy and Pharmacology. 2, 377-392.

Bouzenna, H, Samout, N, Dhibi, S, Rjeibi, I, Elfeki, A, Hfaiedh, N.(2016). Protective Effects of Pinushalepensis L. Essential Oil on Aspirin-induced Acute Liver and Kidney Damage in Female Wistar Albino Rats.J OleoSci.65(8), 701-12.

Bouzenna, H., Hfaiedh, N., Bouaziz, M., Giroux-Metges, M-A., Elfeki, A., Talarmin, H. (2017). Cytoprotective effects of essential oil of PinushalepensisL. against aspirin inducedtoxicity in in rats. Biomed Pharmacother. 2016; 83: 1327-1334.

Rosine, C., Momo, D. (2009). Évaluation de l’activité antidermatophytique des extraits auméthanol et fractions d’Acalyphammahirtum (melastomatacees). Université de Dschang –Master en biochimie clinique et pharmacologie.

Karumi, Y., Onyeyili, PA., Ogugbuaja., VO. (2004). Identification of active principales of M. balsamina (Balsam apple) leaf extract. J Med Scien.4, 179 – 182.

Wong, CC.,Li, HB., Cheng, KW. ,et al . (2006). Systematic survey of antioxidant activity of 30chinese medicinal plants using the ferric reducing antioxydant power assay. Food Chem. 97, 705 – 11.

Zhishen, J., Mengcheng, T.,Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem.64, 555 – 559.11.Ba, K., Tine, E., Destin, J., Cissé, N., Thonart P. (2010). Etude comparative descomposés phénoliques, du pouvoir antioxydant de différentes variétés de sorghosénégalais et des enzymes amylolytiques de leur malt. Biotechnol. Agron. Soc. Environ.14(1), 131-139.

Blois, M.S. (1958).Antioxydant determinations by the use of a stable free radical.Nature.26, 1199 – 1200.

Yen, GC.,Chen HY. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem.43, 27 – 37.

Ngameni, B., Kuete, V., Simo, I.K, Mbaveng, A.T, AwoussongP.K, PatnamR., Roy R., Ngadjui B.T. (2009). Antibacterial and antifungal activities of the crude extract and compounds from Dorsteniaturbinata (Moraceae). South African J Botany.75, 256-261.

Benaziza. (2017). Contribution à l’étude phytochimiqueet l’activité antioxydante des extraits de feuilles et de cônes de Pinushalpensis(Pin d’Alep).

Bourgou, S., Pichette, A., Lavoie, S., Marzouk, B., Legault, J. (2012). Terpenoids isolated from Tunisian Nigella sativa L. essential oil with antioxidant activity and the ability to inhibit nitric oxide production. FlavourFragrJ. 27, 69–74.

Trabelsi, N., Megdiche, W., Ksouri, R., Falleh, H., Oueslati, S., Soumaya, B., Abdelly, C. (2010). Solvent effects on phenolic contents and biological activities ofthe halophyte Limoniastrummonopetalumleaves. LWT-Food Science and Technology.43(4), 632-639.

Nigro, H. (2016). Contribution à l’étude phytochimique et à l’effet antioxydant des extraitsde Pinushalepensis. Mémoire En vue de l’obtention du Diplôme de MASTER.78p.

Talbi, H., Boumaza, KM., Talbi, J., Hilalai A. (2015). Evaluation de l’activitéantioxydante et la composition physic-chimique des extraits méthanolique et aqueuxde la Nigellasativa L. Mate-Environ. Sciences.6(4), 1111-1117.

Feng, S., Zeng, W., Luo, F, Zhao., J, Yang, Z., Sun, Q. (2010).Food Sci.Biotechnol.19(1), 35-41.

Fekih, N. (2014). Proprietes chimiques et biologiques des huiles essentielles de trois espèces du genre pinus poussant en algerie. Thèse en vue de l’obtention du diplôme de Doctorat Es-Sciences en Chimie.Option: Chimie Organique Appliquée.


No Supplementary Material available for this article.
Article Views      : 0
PDF Downloads : 0