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Abstract

Aims: To assess the cytotoxicity, thrombolytic, anthelmintic and antioxidant activity of mehanol extract of Litsea monopetala (Family: Lauraceae) leaves in laboratory using in vitro methods.

Methods: Cytotoxicity test was done by brine shrimp lethality bioassay where the extract concentration was 400, 200, 100, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125 (µg/ml). In vitro thrombolytic activity of Litsea monopetala was performed by clot lysis method using extract concentration 2.5, 5, 10 and 20 (mg/mL) in saline water. Anthelmintic activity test was done by using adult earthworms where 10, 20, 40, 60, 80 (mg/ml) extract concentration were used. Finally antioxidant activity was determined by total phenolic content determination using Folin-Ciocalteu reagent.

Result: The Litsea monopetala extract showed cytotoxic activity against brine shrimp nauplii and LC50 value was 41.05(µg/ml) and the investigated thrombolytic activity in our research was 9.52, 9.49, 13.64 and 17.50 %   respectively as % of clot lysis. The paralysis time were at 76.75 min, 60 min, 51.75 min, 44.5 min and 64.5 min and death were at 90.50min, 63.75min, 55.50min, 44.75min and 71min. respectively. The Litsea monopetala extract displayed significant antioxidant activity which was 20.75 (mg of GAE / gm) of extracts.

Conclusions: The activities observed could be attributed to presence of some of the phytochemicals which have been related with cytotoxic, thrombolytic, anthelmintic and antioxidant property.

Keywords: Litsea monopetala cytotoxic thrombolytic anthelmintic and antioxidant activity.

References

  1. Raju GS, Moghal MMR, Dewan SMR, Amin MN, Billah MM. Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic, and antimicrobial activities of Solanum violaceum Ortega. Avicenna Journal of Phytomedicine. 2013, 3(4): 313-320.
  2. Baul S, Amin MN, Hussain MS, Mukul MEH, Millat MS, Rashed MSU et al. Phytochemical Nature and Pharmacological Evaluation of Chloroform Extract of Pandanus fascicularis L. (Fruits) An in vivo Study. Journal of Bioanalysis & Biomedicine 2017, 9(4): 223-228.
  3. Dewan SMR, Amin MN, Adnan T, Uddin SMN, ShahidUd-Daula AFM, Sarwar G et al. Investigation of analgesic potential and in vitro antioxidant activity of two plants of Asteraceae family growing in Bangladesh. Journal of Pharmacy Research. 2013, 6(6): 599-603.
  4. Uddin SMN, Amin MN, Shahid-Ud-Daula AFM, Hossain H, Haque MM, Rahman MS. Phytochemical screening and study of antioxidant and analgesic potentials of ethanolic extract of Stephania japonica Linn. Journal of Medicinal Plant Resrearch, 2014; 8(37): 1127-1133.
  5. Amin MN, Dewan SMR, Noor W, Shahid-Ud-Daula AFM. Characterization of chemical groups and determination of total phenolic content and in-vitro antioxidant Activities of ethanolic extract of Ocimum sanctum leaves growing in Bangladesh. European Journal of Experimental Biology, 2013; 3(1): 449-454.
  6. Amin MN, Banik S, Ibrahim M, Moghal MMR, Majumder MS, Siddika R. A Study on Ardisia solanacea for Evaluation of Phytochemical and Pharmacological Properties. International Journal of Pharmacognosy and Phytochemical Research 2015; 7(1); 8-15.
  7. Tanna MTH, Amin MN, Ibrahim M, Mukul MEH, Kabir A. Evaluation of antioxidants, membrane stabilizing, cytotoxic and anthelmintic activity with phytochemical screening of Chromolaena odorata: A medicinal shrub. International Journal of Pharmacy, 2016, 6(1):53-61.
  8. Amin MN, Siddiqui SA, Uddin MG, Ibrahim M, Uddin SM et al. Increased Oxidative Stress, Altered Trace Elements, and Macro-Minerals Are Associated with Female Obesity. Biol Trace Elem Res, 2020, 197:384–393.
  9. Amin MN, Hussain MS, Sarwar MS, Rahman Moghal MM, Das A, Hossain MZ, et al.
  10. How the association between obesity and inflammation may lead to insulin resistance and cancer. Diabetes Metab Syndr. 2019; 13(2):1213-1224.
  11. Tarequl Islam , Md Saddam Hussain, Mohammad Nurul Amin, Ashraful Mahmud Tuhin. Evaluation of psychopharmacological and neurosafety profile of Swas Kas Chintamani Ras (SKC) in Swiss-Webster mice. Avicenna J Phytomedicine, 2018; 8(1):85-95.
  12. Amin MN, Liza KF, Sarwar MS, Ahmed J, Adnan MT, Chowdhury MI. et al. Effect of lipid peroxidation, antioxidants, macro minerals and trace elements on eczema. Arch Dermatol Res, 2015; 307(7):617-23.
  13. Ghosh A, Banik S, Amin MN, Ahmed J. Evaluation of antinociceptive, antihyperglycemic, and membrane stabilizing activities of Garcinia lancifolia Roxb. J Tradit Complement Med., 2018 ;8(2):303-307.
  14. Muktadir MHA, Islam MA, Amin MN, Ghosh S, Siddiqui SA, Debnath D. Nutrition transition - Pattern IV: Leads Bangladeshi youth to the increasing prevalence of overweight and obesity. Diabetes Metab Syndr., 2019; 13(3):1943-1947.
  15. Islam MA, Amin MN, Siddiqui SA, Hossain MP, Sultana F, Kabir MR. Trans fatty acids and lipid profile: A serious risk factor to cardiovascular disease, cancer and diabetes.
  16. Diabetes Metab Syndr., 2019; 13(2):1643-1647.
  17. Baul S, Amin MN, Hussain MS, Mukul MEH, Millat MS, Rashed MSU et al. Phytochemical Nature and Pharmacological Evaluation of Chloroform Extract of Pandanus fascicularis L. (Fruits) An in vivo Study. Journal of Bioanalysis & Biomedicine 2017, 9(4): 223-228.
  18. Uddin SMN, Amin MN, Shahid-Ud-Daula AFM, Hossain H, Haque MM, Rahman MS. Phytochemical screening and study of antioxidant and analgesic potentials of ethanolic extract of Stephania japonica Linn. Journal of Medicinal Plant Resrearch, 2014; 8(37): 1127-1133.
  19. Amin MN, Dewan SMR, Noor W, Shahid-Ud-Daula AFM. Characterization of chemical groups and determination of total phenolic content and in-vitro antioxidant Activities of ethanolic extract of Ocimum sanctum leaves growing in Bangladesh. European Journal of Experimental Biology, 2013; 3(1): 449-454.
  20. Amin MN, Banik S, Ibrahim M, Moghal MMR, Majumder MS, Siddika R. A Study on Ardisia solanacea for Evaluation of Phytochemical and Pharmacological Properties. International Journal of Pharmacognosy and Phytochemical Research 2015; 7(1); 8-15.
  21. Raju GS, Moghal MMR, Dewan SMR, Amin MN, Billah MM. Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic, and antimicrobial activities of Solanum violaceum Ortega. Avicenna Journal of Phytomedicine. 2013, 3(4): 313-320.
  22. Tanna MTH, Amin MN, Ibrahim M, Mukul MEH, Kabir A. Evaluation of antioxidants, membrane stabilizing, cytotoxic and anthelmintic activity with phytochemical screening of Chromolaena odorata: A medicinal shrub. International Journal of Pharmacy, 2016, 6(1): 53-61.
  23. Raju GS, Moghal MMR, Dewan SMR, Amin MN, Billah MM. Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic, and antimicrobial activities of Solanum violaceum Ortega. Avicenna Journal of Phytomedicine. 2013, 3(4): 313-320.
  24. Raju GS, Moghal MMR, Dewan SMR, Amin MN, Billah MM. Characterization of phytoconstituents and evaluation of total phenolic content, anthelmintic, and antimicrobial activities of Solanum violaceum Ortega. Avicenna Journal of Phytomedicine. 2013, 3(4): 313-320.
  25. Su JX, Wang W, Zhang LB, Chen ZD. Phylogenetic placement of two enigmatic genera, Borthwickia and Stixis, based on molecular and pollen data, and the description of a new family of Brassicales, Borthwickiaceae. Taxon, 2006; 61(3): 601-611.
  26. Reddy ARK, Grace JR. In vitro Evaluation of Antioxidant Activity of Brugeiera Gymnorrhiza and Aegialitis Rotundifolia. Med Aromat Plants. 2016; 5:231.
  27. Sikder MAA, Kaisar MA, Rashid MA, Millat MS, Sultana A. In vitro membrane stabilizing activity, total phenolic content, cytotoxic, thrombolytic and antimicrobial activities of Calliandrasurinamensis (Wall.). J. Pharmacog. Phytochem, 2012; 1(3): 45-50.
  28. Å kerget M, Kotnik P, Hadolin M, HraÅ¡ AR, SimoniÄ M, Knez Ž. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food chemistry, 2005; 89(2): 191-198.
  29. Rahaman MZ, Akhter S, Islam MR, Begum S, Mondal KK, Mottakin M, et al. Assessment of thrombolytic, antioxidant and analgesic properties of a medicinal plant of Asteraceae family growing in Bangladesh. Discovery Phytomedicine, 2020; 7(1): 47-52.
  30. McChesney JD, Venkataraman SK,Henry JT. Plant natural products: back to the future or into extinction?. Phytochemistry. 2015; 68(14):2015-22.
  31. Cartea ME, Francisco M, Soengas P, Velasco P. Phenolic compounds in Brassica vegetables. Molecules. 2011; 16(1):251-280.
  32. Rahman AHMM, Sultana N, Islam AKMR, Zaman ATMN. Study of medical ethno-botany at the village Genda under Savar Upazilla of District Dhaka, Bangladesh. Online International Journal of Medicinal Plants Research. 2013;2(1): 18-31.
  33. Montuschi P, Barnes JP, Roberts JL. Isoprostanes: markers and mediators of oxidative stress. The FASEB Journal. 2004; 18(15): 1791-1800.
  34. Roja G, Rao C. Anticancer compounds from tissue cultures of medicinal plant. Journal of Herbs.Spices and Medicinal Plants, 2000; 7:71-102.
  35. Bekker J, Ploem S, de Jong KP. Early hepatic artery thrombosis after liver transplantation: A systematic review of the incidence, outcome and risk factors. Am J Transplant. 2009;9(4):746-757.
  36. Anwar SM, Khan IN, Sarkar MM, Barua S, Kamal ATMM, Hosen MZ. Thrombolytic & cytotoxic effect of different herbal extracts. IJPSR. 2011; 2(12):3118-3121.
  37. Sherwani SK, Bashir A, Haider SS, Shah MA, Kazmi SU. Thrombolytic potential of aqueous and methanolic crude extracts of Camellia sinensis (Green Tea): In vitro study. Journal of Pharmacognosy and Phytochemistry. 2013; 2(1):125-129.
  38. Giuseppina B, Cristiana L, Guido L, Piero C, Antonio LA, Daniele R. Therapeutic effect of diagnostic ultrasound on enzymatic thrombolysis. An in vitro study on blood of normal subjects and patients with coronary artery disease, Journal of Thrombosis and Haemostasis, 91, 2004, 1078-1083.
  39. Steinmann P, Keiser J, Bos R, Tanner M, Utzinger J. Schistosomiasis and water resources development: Systemic review, meta-analysis, and estimates of people at risk. Lancet Infect. Dis. 2006; 6:411-425.
  40. Hasan H, Azad MSL, Islam MZ, Rahman SM, Islam MR, Rahman S, Rahmatullah M. Antihyperglycemic activity of methanolic extract of Litsea monopetala (Roxb.) Pers. leaves. Adv Nat Appl Sci. 2014; 8:51-55.
  41. Hossain S, Kader G, Nikkon F, Yeasmin T. Cytotoxicity of the rhizomes of medicinal plants. Asian Pac J Trop Biomed. 2012; 2(2):125–127.
  42. Prasad S, Kashyap RS, Deopujari JY, Purohit HJ, Taori GM, Daginawala HF. Development of an in vitro model to study clot lysis activity of thrombolytic drugs. Thromb J. 2006; 4(14):1–4.
  43. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen JE, Nichols DE, McLaughlin JL. Brine shrimp: A convenient general bioassay for active plants constituents. J Med Plant Res. 1982;45:31–34.
  44. Vital PG, Rivera WL. Antimicrobial activity, cytotoxicity, and phytochemical screening of Voacangaglobosa (Blanco) Merr. leaf extract (Apocynaceae) Asian Pac J Trop Med. 2011;4(10):824–828.

How to Cite

Mohammad, N. (2021). A study on Litsea monopetala for evaluating its pharmacological properties. Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 8(1). https://doi.org/10.15562/phytomedicine.2021.159
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