Detection of Secondary Metabolites Using HPTLC and GC-MS Analysis and Assessment of Pharmacological Activities of Phoenix loureiroi Kunth (Arecaceae) Ethanolic Leaves Extract in the Management of Pyrexia, Pain and Inflammation

Sumanta Mondal , Prasenjit Mondal, Suvendu Kumar Sahoo, Naresh Panigrahi, Sara Almas, Kausik Bhar, Suman Acharyya

Sumanta Mondal
GITAM (Deemed to be University). Email: mondalresearch@gmail.com

Prasenjit Mondal
Vaageswari College of Pharmacy

Suvendu Kumar Sahoo
GITAM (Deemed to be University)

Naresh Panigrahi
GITAM (Deemed to be University)

Sara Almas
GITAM (Deemed to be University)

Kausik Bhar
Bengal School of Technology

Suman Acharyya
Online First: March 08, 2021 | Cite this Article
Mondal, S., Mondal, P., Sahoo, S., Panigrahi, N., Almas, S., Bhar, K., Acharyya, S. 2021. Detection of Secondary Metabolites Using HPTLC and GC-MS Analysis and Assessment of Pharmacological Activities of Phoenix loureiroi Kunth (Arecaceae) Ethanolic Leaves Extract in the Management of Pyrexia, Pain and Inflammation. Discovery Phytomedicine 8(2). DOI:10.15562/phytomedicine.2021.163

The present research work was carried out the High Performance Thin Layer Chromatography (HPTLC) and Gas Chromatography-Mass Spectrometry (GC-MS) analysis and assessment of pharmacological activities of Phoenix loureiroi Kunth (Arecaceae) ethanolic leaves extract at doses of 200, 400 and 600 mg/kg, body weight, per os. Preliminary phytochemical screening, HPTLC and GC-MS studies were carried out according to the standard methods. The acute toxicity studies were conducted on Swiss albino mice as per Organization for Economic Cooperation and Development (OECD) guidelines 420. For the screening of analgesic activity, writhing test was conducted for peripheral analgesic activity whereas tail immersion test was conducted for central analgesic activity. Antipyretic activity was performed by using the yeast induced hyperpyrexia method and for the screening of anti-inflammatory activity carrageenan-induced rat paw edema method was used. Preliminary phytochemical screening of the ethanol extract of Phoenix loureiroi leaves (EEPLL) contains sterols, flavonoids, saponins, proteins, reducing sugar, tannins, and phenolic compounds. The HPTLC analysis method employed in this work resulted in good peak shape and enabled good resolution of quercetin present in the extract and GC-MS analysis showed a total of 25 peaks and led to the identification of 22 different phytoconstituents in the ethanolic extract. Lethal Dose 50 (LD50) was above 2,000 mg/kg and no death was recorded. The prevailing study demonstrated that EEPLL possesses widespread analgesic, antipyretic and anti-inflammatory effects in dose dependent manner. It can be concluded that the ethanolic extract from Phoenix loureiroi leaves possesses promising analgesic, antipyretic and anti-inflammatory activities.


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