Skip to main content Skip to main navigation menu Skip to site footer

Phytochemical study and anti-nutritional factors in stems of Dioscorea praehensilis benth (Dioscoreaceae)

  • Florent Biduaya Mukeba ,

Abstract

The objective of this study was to identify and assay phytochemical compounds and various biological macromolecules of the tender stems of Dioscorea praehensilis benth and evaluate their antioxidant activity and to compare the content of oxalates and cyanogenetic glucosides between raw and cooked tender stems. The plant collection and identification, phytochemical evaluation: phytochemical screening, preliminary (qualitative) analyses and in vitro assays. Phytochemical screening was performed by qualitative methods. The estimation of the content of secondary metabolites was evaluated by spectrophotometry-UV. Antioxidant activity was evaluated using the ABTS and DPPH assays and preliminary composition by the gravimetric method. The results obtained show that the stems of Dioscorea praehensilis are devoid of certain important chemical groups, the flavonoids were not detected and they were rich in total polyphenols (17.22 ± 0.16), tannins (19.32 ± 0.52) and anthocyanins (25.22 ± 0.04). Our extracts showed a lower antioxidant activity than that of positive controls. The samples are rich in carbohydrates and fiber, with low levels of proteins, lipids and ash. Dioscorea praehensilis has a high toxicity in HCN, but after a good cooking of about 1 hour, 99.97% of the cyanide are eliminated and does not have many oxalates. The results obtained show that Dioscorea praehensilis has a high dietary value and can therefore be used as a nutritive food.

References

  1. Bayiha, G., Temple, L., Mathe, S., Nesme, T. (2019). Typologie et perspective d’évolution de l’agriculture biologique au Cameroun. Cahiers Agricultures, 28, 3. https://doi.org/10.1051/cagri/2019003.
  2. FAO (2010). Développer des villes plus vertes en République Démocratique du Congo. Programme de la FAO pour l’horticulture urbaine et périurbaine. 00153 Rome, Italie. 35 p.
  3. Minengu, J.D.D., Ikonso, M., Mawikiya, M. (2018). Agriculture familiale dans les zones péri-urbaines de Kinshasa : analyse, enjeux et perspectives (synthèse bibliographique). Revue Africaine d’Environnement et d’Agriculture, 1(1), 60-69.
  4. Asiedu, R., Sartie, A. (2010). Crops that feed the World 1. Yams Yams for income and food security. Food Security, 2: 305–315.
  5. Sesay, L., Norman, P.E., Massaquoi, A., Kobba, F., Allieu, A.P,, Gboku, M.L., Fomba, S.N. (2013). Assessment of farmers’ indigenous knowledge and selection criteria of yam in Sierra Leone. Sky Journal of Agricultural Research, 2(1): 1 – 6.
  6. Adeniyan, O.N., Owolade, O.F. (2012). Comparative performance of improved white yam (Dioscorea rotundata) genotypes in the rainforest belt of Southwest Nigeria. International Research Journal of Agricultural Science and Soil Science, 2(4): 127-132.
  7. Dansi, A., Orobiyi, M., Dansi, P., Assogba, A., Sanni, & Akpagana, K. (2013). Sélection de sites pour la conservation in situ des ignames sauvages apparentées aux ignames cultivées : cas de Dioscorea praehensilis au Bénin. International Journal of Biological and Chemical Sciences, 7(1): 60-74.
  8. Dumont, R., Dansi, A., Vernier, Ph., Zoundjihékpon, J. (2005). Biodiversité et domestication des ignames en Afrique de l’Ouest : Pratiques traditionnelles conduisant à Dioscorea rotundata, 119 p.
  9. Chaïr, H., Perrier, X., Agbangla, C., Marchand J.L., Dainou, O., Noyer, J.L. (2005). Use of cpSSRs for the characterisation of yam phylogeny in Benin. Genome, 48: 674– 684.
  10. Mignouna, H.D., Dansi, A. (2003). Yam (Dioscorea sp.) domestication by the Nago and Fon ethnic groups in Benin.Genetic Resources and Crop Evolution 50(5): 519-528.
  11. Scarcelli, N., Tostain, S., Mariac, C., Agbangla, C., Daïnou, O., Berthaud, J., Pham, J-L. (2006) . Genetic nature of yams (Dioscorea sp.) domesticated by famers in Benin (West Africa). Genetic Resources and Crop Evolution, 53(1): 121-130.
  12. Tamiru, M., Becker, C.H., Maass, B.L. (2008). Diversity, distribution and management of yam landraces (Dioscorea spp.) in Southern Ethiopia. Genetic Resources and Crop Evolution, 55(1): 115- 131.
  13. Ortiz, P.L., Berjano, R., Talavera, M., Arista, M. (2009). The role of resources and architecture in modeling floral variability for the monoecious amphicarpic emex spinosa (Polygonaceae). American Journal of Botany, 96(11): 2062–2073.
  14. Maxted, N., White, K., Valkoun, J., Konopka, J., Hargreaves, S. (2008). Towards a conservation strategy for Aegilops species. Plant Genetic Resources: Characterization and Utilization, 6(2):126-141.
  15. Chatzav, M., Peleg, Z., Ozturk, L., Yazici, A., Fahima, T., Cakmak, I., Saranga, Y. (2010). Genetic diversity for grain nutrients in wild emmer wheat: potential for wheat improvement. Annals of Botany, 105 (7):1211- 1220.
  16. Luse, R.A. (1975). The Role of Grain Legume in Tropical Nutrition, Proc. 1st African Nutrition Congress,
  17. Bruneton, J. (1999). Pharmacognosie, Phytochimie et Plantes médicinales. Edition Technique et Documentation-Lavoisier. Paris 3e édition, 421- 499.
  18. Harborne, JB, Baxter, H. (1995). Phytochemical dictionary: a handbook of bioactive compounds from plants. 4 John St, Taylor & Francis Ltd, London.
  19. Kokate, CK, Purohit, AP, Gokhale, SB. (1981). Test book of Pharmacognosy. Nirali Prakashan, Pune, 2003.
  20. Gutfinger, T. Polyphenols in olive oils. J Am Oil Chem Soc. 58:966-968.
  21. Querttier-Deleu, C., Gressier, B., Vassseur, J., Dine, T., Brunet, C., Luyckx, M.C., Cazin, J.C., Bailleu, F., Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. J Ethnopharmacol. 72:35-42
  22. Zhang, S.Y., Zheng, C.G., Yan, X.Y., Tian, W.X. (2008). Low concentration of condensed tannins from catechu significantly inhibits fatty acid synthase and growth of MCF-7 cells. Biochem Biophys Com.v371:654-658.
  23. Sun, B., Ricardo-da-Silva, J.M., Spranger. (1998). Critical factors of vanillin assay for catechins and proanthocyanidins. J Agric Food Chem. 46(10):42674274.
  24. Adedapo, A.A., Jimoh, F.O., Koduru, S. et al. (2008). Antibacterial and antioxidant properties of the methanol extracts of the leaves and stems of Calpurnia aurea . BMC Complement Altern Med 8, 53. https://doi.org/10.1186/1472-6882-8-53
  25. Mukeba, F.B., Bopopi, J.M., Mayangi, M.M., Kikweta, C., Bamba, J.V., Kitwa, F., Mutwale, P.K., Ngbolua, K.J.P., Ngombe, N.K., Mpiana, P.T., Mbemba, T.F. (2020). Selenium content, antibacterial, antioxidant and anti-sickling activities of Zanthoxylum gilletii (De Wild) P.G. Waterman (Rutaceae). Discovery Phytomedicine 7(4): 145-154. DOI: 10.15562/phytomedicine.2020.141
  26. Mbemba, F., Remacle, J. (1992). Inventaire et composition chimique des aliments et denrées alimentaires traditionnels du Kwango-Kwilu au Zaïre. Presses universitaires de Namur. Namur, p : 80.
  27. Degroote. (1965). Analyses des macromolécules par les méthodes physico-chimique/phytochimie. 1964, p 66-72
  28. Vervack, W. (1982). Analyse des aliments. Laboratoire de Biochimie de la Nutrition. Université Catholique de Louvain-la-Neuve/Belgique. 2è Partie. pp.10-45
  29. AOAC. (1970). Official methods of analysis of the association of official analytical chemists, 11th Ed. Washington DC.
  30. Sadasivam, S., Manickam, A. (1996). Biochemical methods for agricultural science. Wiley Eastern Limited, New Delhi.18.
  31. Mbemba, F. (2013). Aliments et denrées alimentaires traditionnels de Bandundu en R.D.Congo. ed. L’Harmattan, Paris. French
  32. Makengo, K.G., Bongeli, A., Mbemba, F.T. (2015). Contribution à l’étude de la valeur biologique d’un aliment de complément à base des ressources alimentaires locales: Cas de la Vamine. Ann Fac. Sci (Université de Kinshasa). 1:43-51. French
  33. AOAC. (1984). Official methods of analysis (p. 500, 14th ed.). Arlington, VA, USA: Association of Official Analytical Chemists.
  34. Oke, O.L. (1966). Chemical studies on some Nigerian Vegetables. Trop. Sci. Trop. Sci. 8(3): 128-132.
  35. Bukatuka, F.C., Ngombe, K.N., Mutwale, K.P., Moni, B.M., Makengo, K.G., Pambu, L.A., Bongo, N.G., Mbombo M.P., et al. (2016). Bioactivity and Nutritional Values of Some Dioscorea Species Traditionally Used as Medicinal Foods in Bandundu, DR Congo. European Journal of Medicinal Plants 14(1): 1-11, Article no.EJMP.25124. ISSN: 2231-0894, NLM ID: 101583475.
  36. Sherman, H.C. (1952). Chemistry of Food and Nutrition. The MacMilan Company, New York.
  37. Bray, T.M, Taylor CG. (1993). Tissue glutathione, nutrition, and oxidative stress. Can J Physiol Pharmacol, 71 and 746-5.
  38. AOAC. (1980). Anonymous Official methods of analysis, Association of Official Analytical Chemist, Washington DC, 10th Edition.
  39. Hossain, M.A., Nagooru, M.R. (2011). Biochemical profiling and total flavonoids and contents of leaves crude extract of endemic medicinal plants Corydyline terminalis L Kunth. Pharmacognosy Journal, 3(24): 25-29.
  40. Suresh, S.N., Nagarajan, N. (2009). Preliminary phytochemical and antimicrobial activity analysis of Begonia malabarica Lam. J. Basic appl. biol, 3(1&2): 59-61.
  41. Saidu, A.N., Mann, A., Onuegbu, C.D. (2012). Pytochemical screening and hypoglycemic effect of aqueous Blighia sapida root bark extract on normoglycemic albino rats. Bri J Pharmaceu Res, 2: 89-97.
  42. Sasikumar, J.M., Maheshu, V., Aseervatham, G.S.B., Darsini, D.T.P. (2010). In vitro antioxidant activity of Hedyotis corymbosa (L) Lam aerial plants. Indian J Biochem and Biophy; 47: 49-52.
  43. Bhandari, M.R., Kawabata, J. (2004). Organic acid, phenolic content and antioxidant activity of wild yam (Dioscorea spp.) tubers of Nepal. Food Chemistry. 88(2):163168.
  44. Subasini, U., Thenmozhi, S., Sathyamurthy, D., Vetriselvan, S., Rajamanickam, G.V., Dubey, G.P. (2013). Pharmacognostic and phytochemical investigations of Dioscorea bulbifera L Int. J. of Pharm. & Life Sci. (IJPLS), 4(5): 2693-2700
  45. Floegel, A., Kim, D.O., Chung, S.J., Koo, S.I., Chun, O.K. (2011). Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Comp Anal. 24(7):10431048.
  46. Ngbolua, K.N., Bishola, T.T., Mpiana, P.T., Mudogo, V., Tshibangu, D.S.T., Ngombe, K.N., Tshilanda, D.D., Baholy, R. (2014a). In vitro antisickling and free radical scavenging activities of Pentaclethra macrophylla Benth (Fabaceae). J Advancement Med Life Sci. V1I2. DOI: 10.15297/JALS.V1I2.03
  47. Ngbolua, K.N., Bishola, T.T., Mpiana, P.T., Mudogo, V., Tshibangu, D.S.T., Ngombe, K.N., Ekutsu, E.G., Gbolo, B.Z., Kabena, N.O. (2014b). Ethno-pharmacological survey,in vitro antisickling and free radical scavenging activities of Carapa procera DC. Stem bark (Meliaceae). Nova J Med Biol Sci. 2(2):01-14.
  48. Ngbolua, K.N., Bishola, T.T., Mpiana, P.T., Mudogo, V., Tshibangu, D.S.T., Ngombe K.N., Ekutsu, E.G., Tshilanda, D.D., Gbolo, B.Z., Mwanangombo, D.T., Fatiany, P.R., Baholy, R. (2014c). Ethno-botanical survey, in vitro antisickling and free radical scavenging activities of Garcinia punctata Oliv. (Clusiaceae). J Adv Bot Zool.; V1I2. DOI: 10.15297/JABZ.V1I2.04.
  49. Ngbolua, K.N., Mubindukila, N., Mpiana, P.T., Tshibangu, D.S.T., Masengo, C.A., Nzongola, K., Baholy, R., Fatiany, P.R. (2014d). Phytochemical screening, antibacterial and antioxidant activities of Anthocleista liebrechtsiana Wild & T. Durand (Gentianaceae) originated from Democratic Republic of the Congo. J Adv Med Life Sci.V1I3. DOI:10.15297/JALS.V1I3.04.
  50. Savage, M. (2000). Effect of cooking on the soluble and insoluble oxalate content of some New Zealand foods. J. Food Compos. Anal. 13(3): 201-206.

How to Cite

Mukeba, F. B. (2023). Phytochemical study and anti-nutritional factors in stems of Dioscorea praehensilis benth (Dioscoreaceae). Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 8(3). https://doi.org/10.15562/phytomedicine.2021.178

HTML
62

Total

Share

Search Panel