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Effects of Cotinus coggygria extract on human blood T and NK cells activity in vitro


It is an important research area to define the bioactivities of plant fractions and to create appropriate utilization areas for them. Although Cotinus coggygria, which is widely used in folk medicine, which grows from Europe to the center of China, has a wide variety of bioactivities, there is limited information about its influence on the immune system.

The anti-proliferative effects of Cotinus coggygria extract on stimulated peripheral blood mononuclear cells (PBMC) were determined by CFSE dilution method. The effects of the extract on peripheral blood T and natural killer (NK) cells were followed by their proliferative responses. Annexin V / PI staining method was used to evaluate the apoptotic effects of the extract on PBMCs. The effects of the extracts on expression levels of IL-1?, IL-2, IL-4 and IL-10 genes were investigated.

Effect of Cotinus coggygria on the proliferation of T cells and NK cell subsets were decreased in a dose dependent manner. Anti-proliferative effect of Cotinus coggygria extract on stimulated PBMCs was not directly related to its apoptotic properties, it also changes the cytokine genes expression on PBMCs. 

Our study suggests that Cotinus coggygria methanol extract, whose anti-inflammatory effects have not been investigated yet, may be a pioneer in the identification of new molecules with anti-inflammatory properties and the development of related therapies.


  1. Nicholson D, Nicholson LB. A simple immune system simulation reveals optimal movement and cell density parameters for successful target clearance. Immunology. 2008;123(4):519-27 DOI: 10.1111/j.1365-2567.2007.02721.x
  2. Chan S, Correia-Neves M, Dierich A, Benoist C, Mathis D. Visualization of CD4/CD8 T cell commitment. J Exp Med. 1998;188(12):2321-33 DOI: 10.1084/jem.188.12.2321
  3. Coffman RL. Origins of the T(H)1-T(H)2 model: a personal perspective. Nat Immunol. 2006;7(6):539-41 DOI: 10.1038/ni0606-539
  4. Balomenos D, Martinez AC. Cell-cycle regulation in immunity, tolerance and autoimmunity. Immunol Today. 2000;21(11):551-5 DOI: 10.1016/s0167-5699(00)01748-5
  5. Rajan TV. The Gell-Coombs classification of hypersensitivity reactions: a re-interpretation. Trends in Immunology. 2003;24(7):376-9
  6. Mukherjee M, Nair P. Autoimmune Responses in Severe Asthma. Allergy Asthma Immunol Res. 2018;10(5):428-47 DOI: 10.4168/aair.2018.10.5.428
  7. Bragazzi NL, Watad A, Brigo F, Adawi M, Amital H, Shoenfeld Y. Public health awareness of autoimmune diseases after the death of a celebrity. Clin Rheumatol. 2017;36(8):1911-7
  8. Ahluwalia B, Magnusson MK, Isaksson S, Larsson F, Öhman L. Effects of Aloe barbadensis Mill. extract (AVH200®) on human blood T cell activity in vitro. Journal of Ethnopharmacology. 2016(179):301-10
  9. Miroslav Novakovi? IV, Pe?a Jana?kovi?, Mar?na,Sokovi?, Anka Filipovi?, Vele Teševi? and Slobodan Milosavljevi?. Chemical composition, antibacterial and antifungal activity of the essential oils of Cotinus coggygria from Serbia. J Serb Chem Soc. 2007;72(11)
  10. Matic S, Stanic S, Mihailovic M, Bogojevic D. Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential. Saudi J Biol Sci. 2016;23(4):452-61 DOI: 10.1016/j.sjbs.2015.05.012
  11. Westenburg HE, Lee KJ, Lee SK, Fong HH, van Breemen RB, Pezzuto JM, et al. Activity-guided isolation of antioxidative constituents of Cotinus coggygria. J Nat Prod. 2000;63(12):1696-8 DOI: 10.1021/np000292h
  12. Wang G, Wang JJ, Du L, Fei L, To ST. Inhibitory Kinetics and Mechanism of Flavonoids Extracted from Cotinus coggygria Scop. Against Glioblastoma Cancer. Nutr Cancer. 2016;68(8):1357-68 DOI: 10.1080/01635581.2016.1225105
  13. Marcetic M, Bozic D, Milenkovic M, Malesevic N, Radulovic S, Kovacevic N. Antimicrobial, antioxidant and anti-inflammatory activity of young shoots of the smoke tree, Cotinus coggygria Scop. Phytother Res. 2013;27(11):1658-63 DOI: 10.1002/ptr.4919
  14. Patil KR, Mahajan UB, Unger BS, Goyal SN, Belemkar S, Surana SJ, et al. Animal Models of Inflammation for Screening of Anti-inflammatory Drugs: Implications for the Discovery and Development of Phytopharmaceuticals. Int J Mol Sci. 2019;20(18) DOI: 10.3390/ijms20184367
  15. Jenny M, Klieber M, Zaknun D, Schroecksnadel S, Kurz K, Ledochowski M, et al. In vitro testing for anti-inflammatory properties of compounds employing peripheral blood mononuclear cells freshly isolated from healthy donors. Inflamm Res. 2011;60(2):127-35 DOI: 10.1007/s00011-010-0244-y
  16. Seemann S, Zohles F, Lupp A. Comprehensive comparison of three different animal models for systemic inflammation. J Biomed Sci. 2017;24(1):60 DOI: 10.1186/s12929-017-0370-8
  17. Ai W, Li H, Song N, Li L, Chen H. Optimal method to stimulate cytokine production and its use in immunotoxicity assessment. Int J Environ Res Public Health. 2013;10(9):3834-42 DOI: 10.3390/ijerph10093834
  18. Peighambarzadeh F, Najafalizadeh A, Esmaeil N, Rezaei A, Ashrafi F, Ganjalikhani Hakemi M. Optimization of In Vitro Expansion and Activation of Human Natural Killer Cells against Breast Cancer Cell Line. Avicenna J Med Biotechnol. 2020;12(1):17-23
  19. Mire-Sluis AR, Wickremasinghe RG, Hoffbrand AV, Timms AM, Francis GE. Human T lymphocytes stimulated by phytohaemagglutinin undergo a single round of cell division without a requirement for interleukin-2 or accessory cells. Immunology. 1987;60(1):7-12
  20. Gospodinova ZI, Zupko I, Bozsity N, Manova VI, Georgieva MS, Todinova SJ, et al. Cotinus coggygria Scop. induces cell cycle arrest, apoptosis, genotoxic effects, thermodynamic and epigenetic events in MCF7 breast cancer cells. Z Naturforsch C J Biosci. 2020 DOI: 10.1515/znc-2020-0087
  21. Kong X, Hao S, Li C, Wang H, Gao Y, Su F, et al. Protective Effect of Compound Cotinus coggygria Oral Liquid on Mice Acute Liver Injury Induced by D-Galactosamine. Ann Clin Lab Sci. 2019;49(3):361-7
  22. Walter U, Santamaria P. CD8+ T cells in autoimmunity. Current Opinion in Immunology. 2005;17(6)
  23. G.X. Zhang BGX, M. Bakhiet, P. van der Meide, H. Wigzell, H. Link, T. Olsson. Both CD4+ and CD8+ T cells are essential to induce experimental autoimmune myasthenia gravis. J Exp Med. 1996;184(349-356)
  24. Alten R, Gomez-Reino J, Durez P. Efficacy and safety of the human anti-IL-1beta monoclonal antibody canakinumab in rheumatoid arthritis: results of a 12-week, phase II, dose-finding study. BMC Musculoskeletal Disorders. 2011;12(153)
  25. Navarro-Sarabia F, Ariza-Ariza R, Hernandez-Cruz B, Villanueva I. Adalimumab for treating rheumatoid arthritis. The Cochrane Database of Systematic Reviews. 2005;3

How to Cite

Gunes, U., Kucuksezer, U. C., Artun, F. T., Karagoz, A., & Deniz, G. (2023). Effects of Cotinus coggygria extract on human blood T and NK cells activity in vitro. Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 9(1).