Effects of Cotinus coggygria extract on human blood T and NK cells activity in vitro
- DOI: https://doi.org/10.15562/phytomedicine.2022.189  |
- Published: 2023-01-08
It is an important research area to define the bioactivities of plant fractions and to create appropriate utilization areas for them. Although Cotinus coggygria, which is widely used in folk medicine, which grows from Europe to the center of China, has a wide variety of bioactivities, there is limited information about its influence on the immune system.
The anti-proliferative effects of Cotinus coggygria extract on stimulated peripheral blood mononuclear cells (PBMC) were determined by CFSE dilution method. The effects of the extract on peripheral blood T and natural killer (NK) cells were followed by their proliferative responses. Annexin V / PI staining method was used to evaluate the apoptotic effects of the extract on PBMCs. The effects of the extracts on expression levels of IL-1?, IL-2, IL-4 and IL-10 genes were investigated.
Effect of Cotinus coggygria on the proliferation of T cells and NK cell subsets were decreased in a dose dependent manner. Anti-proliferative effect of Cotinus coggygria extract on stimulated PBMCs was not directly related to its apoptotic properties, it also changes the cytokine genes expression on PBMCs.
Our study suggests that Cotinus coggygria methanol extract, whose anti-inflammatory effects have not been investigated yet, may be a pioneer in the identification of new molecules with anti-inflammatory properties and the development of related therapies.