The present study was aimed to detect medicinal compounds of crude ethanolic extract of Elaeocarpus robustus
roxb (EEE) and also it’s cytotoxic activity. The cytotoxic activity of EEE was determined by brine shrimp lethality bioassay. This analysis showed that with increasing concentration cytotoxicity of crude extract increased gradually. The LD50
value of EEE was 82.065±2.00 which was nearly about standard vincristine sulphate LD50
value 69.726±1.52. The chi value of EEE (2.34) with the chi square value of standard vincristine sulphate (2.014) was quite similar and indicates little difference exists between the observed counts and expected counts. Through this research we also detect the presence of alkaloid and tannins by several tests like mayar’s reagent, wagner’s reagent, alkaloidal precipitation, ferric chloride test.