In-vivo antiemetic and antinociceptive, cytotoxic and in-vitro thrombolytic potential of hydro-alcoholic stem and leaves extract of jatropha gossypiifolia.

This study describes the biological investigations of hydro-alcoholic leaves and stem extract of Jatropha gossypiifolia, a plant belonging to the family Euphorbiaceae, to reveal their possible contribution as an antiemetic, antinociceptives, cytotoxic and thrombolytic factor. The leaves and stems of Jatropha gossypiifolia were extracted with methanol. The leaves extracts were used for the observation of thrombolytic potential. Result reveals that the leaves at a concentration of 2.5mg/mL, showed maximum activity (27.255 ± 0.90%), whereas 20 mg/mL sample solution showed the lowest activity (7.722 ± 0.26%), when comparing positive control streptokinase. The extracts were used for the investigation of cytotoxic activity considering vincristine sulfate as a positive control. The leaves and the stems portion have considerable cytotoxic property (LC50 are 0.00532 µg/ml for stems and 0.000109 µg/ml for leaves, respectively) when comparing with positive control. The methanolic extract showed potent antiemetic activity at the highest concentration both at leaves and stem extracts as compared to positive control metoclopramide. Where metoclopramide shows 73% of inhibition, there leaves and the stem portion of the plant shows 90% and 91% of emesis inhibition respectively. By using a standard protocol, the crude methanolic stem and leaves extract of J. gossypiifolia was conducted to attempt for antinociceptives activities, where Diclofenac Na is used as a standard. The crude methanolic extract of leaves and stem exhibit 31.07% and 30.79% writhing inhibition in test animals respectively, which are statistically significant. The results of the study showed that the plant extract has potential thrombolytic, cytotoxic, antiemetic and antinociceptive activities.