Antioxidant, Antibacterial and Cytotoxic activities of Ethanol extract and its different fractions of Sterculia cordata leaves

Mohammad Shah Hafez Kabir

Mohammad Shah Hafez Kabir
1Department of Pharmacy, International Islamic University Chittagong, Chittagong, Bangladesh 2Drug Discovery, GUSTO A Research Group, Chittagong, Bangladesh. Email: mohammadshahhafezkabir@gmail.com
Online First: June 11, 2018 | Cite this Article
Kabir, M. 2018. Antioxidant, Antibacterial and Cytotoxic activities of Ethanol extract and its different fractions of Sterculia cordata leaves. Discovery Phytomedicine 5(3): 26-33. DOI:10.15562/phytomedicine.2018.64

Background: Sterculia cordata is a flowering plant and belongs to the family Malvaceae. The goal of our investigation was to determine the significant medicinal properties of the leaf of this plant.

Methods: Leaves of Sterculia cordata was extracted with pure ethanol, then fractioned with n-hexane, Ethyl acetate, chloroform and methanol. The extract and fractions were tested for secondary metabolites and the determination of total phenol, total flavonoid content and antioxidant activity. The antibacterial screening was done by disk diffusion technique. The cytotoxic bioassay was carried out with the method as described by brine shrimp bioassay.

Results: Alkaloid, flavonoid, terpenoid, and tannins are present in all extracts and fractions of Sterculia cordata leaves. Among all extract and fractions, MFEESC exhibited strong antioxidant activity in both reducing powers. Phenol content was at a range of 3.06±0.34 mg to 45.61 ± 0.43mggallic acid/g, flavonoid content was 25.87±0.20 mg to 36.57±0.34 mg quercetin/g. In antibacterial screening, the moderate zone of inhibition (8-12 mm in diameter) was observed against gram-positive Bacillus subtilis, and more promising zone of inhibition (7.8-14mm in diameter) against gram-negative pseudomonas aeruginosa, Escherichia coli. Bacillus cereus, Staphylococcus aureus, Salmonella paratyphi did not show any sensitivity. In brine shrimp lethality bioassay, chloroform fraction revealed the highest cytotoxic activity having LC50 value 95.23 mg/ml.

Conclusions: The overall results of the study indicated significant Antioxidant, antibacterial and cytotoxic activities of Sterculia cordata  leaves. So this plant deserves further investigation to isolate the active constituents responsible for these activities and to establish the mechanism of action.
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