Evaluation of the analgesic and anti-inflammatory activities of methanolic extracts of the leaves of Averrhoa bilimbi leaves

Mohammad Nurul Amin

Mohammad Nurul Amin
. Email: amin.pharma07@gmail.com
Online First: January 15, 2019 | Cite this Article
Amin, M. 2019. Evaluation of the analgesic and anti-inflammatory activities of methanolic extracts of the leaves of Averrhoa bilimbi leaves. Discovery Phytomedicine 6(1): 12-15. DOI:10.15562/phytomedicine.2019.77

Objectives: This study was aimed to investigate the analgesic and anti-inflammatory activi­ties of crude methanolic extract Averrhoa bilimbi leaves.

Materials and Methods: Methanolic extracts of Averrhoa bilimbi leaves with different concentration were tested for an­algesic activity in mouse model by acetic acid induced writhing and anti-inflammatory effect was tested by carrageenan induced paw edema model

Results: The extract, at 400 mg/kg, showed higher analgesic activity (67.51%) against acetic acid induced pain in mice while the standard ref­erence drug Diclofenac sodium exhibited 64.33% activity at 10 mg/kg dose. The anti-inflammatory effect of the extract was comparable to reference drug Ibuprofen and the effect was sustained for 2-4 hr.

Conclusion: Methanolic extractof Averrhoa bilimbi leaves have moderate analgesic and anti-inflammatory properties.


Baul S, Amin MN, Hussain MS, MEH Mukul, Millat MS, et al. Phytochemical Nature and Pharmacological Evaluation of Chloroform Extract of Pandanus fascicularisL. (Fruits): An in vivo Study. J Bioanal Biomed 2017; 9: 223-228. doi:10.4172/1948-593X.1000183

Alhassan AM, Ahmed QU. Averrhoa bilimbi Linn.: A review of its ethnomedicinal uses, phytochemistry, and pharmacology. J Pharm Bioallied Sci. 2016;8(4):265-271. doi: 10.4103/0975-7406.199342.

Mokhtar SI, Aziz NAA. Antimicribial Properities of Averrhoa bilimbi Extracts at Different Maturity Stages. J Med Microb Diagn 2016; 5:233. doi:10.4172/2161-0703.1000233

Mackeen MM, Ali AM, El-Sharkawy SH, Manap MY, Salleh KM, Lajis NH, et al. Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (Ulam) Pharm Biol. 1997;35:174–8.

Zakaria ZA, Zaiton H, Henie EF, Jais AM, Zainuddin EN. In vitro antibacterial activity of Averrhoa bilimbi L. leaves and fruits extracts. Int J Trop Med. 2007;2:96–100.

Winarti C, Marwati T. Effect of bilimbi leaf extracts on decrease blood pressure. J Pascapanen. 2009;6:54–61.

Igaa H. Topical application of ethanol extract of starfruit leaves (Averrhoa bilimbi Linn.) increases fibroblasts in gingival wounds healing of white male rats. Indones J Biomed Sci. 2012;6:35–9.

Pushparaj P, Tan CH, Tan BK. Effects of Averrhoa bilimbi leaf extract on blood glucose and lipids in streptozotocin-diabetic rats. J Ethnopharmacol. 2000;72:69–76.

Pushparaj PN, Tan BK, Tan CH. The mechanism of hypoglycemic action of the semi-purified fractions of Averrhoa bilimbi in streptozotocin-diabetic rats. Life Sci. 2001;70:535–47

Dewan SMR, Amin MN, Adnan T, Uddin SMN, Shahid- Ud-Daula AFM, Sarwar G, et al. Investigation of analgesic potential and in vitro antioxidant activity of two plants of Asteraceae family growing in Bangladesh. Journal of pharmacy research. 2013; 6(6): 599– 603.

Ibrahim M, Amin MN, Millat S, Raju JA, Hussain S, Sultana F, Islam M, Hasan MM. Methanolic Extract of Peel of Citrus maxima Fruits Exhibit Analgesic, CNS Depressant and Anti-inflammatory Activities in Swiss Albino Mice. BEMS Reports. 2018; 4(1):7-11.

Uddin SMN, Amin MN, Shahid-Ud-Daula AFM, Hossain H, Haque MM, Rahman MS et al. Phytochemical screening and study of antioxidant and analgesic potentials of ethanolic extract of Stephania japonica Linn. J Med Plant Res. 2014; 8(37): 1127-1133.

Deraedt R, Jougney S, Delevalcee F, Falhout M. Release of prostaglandin E and F in an algogenic reaction and its inhibition. Eur J pharmacol. 1980;61(1):1724.

Kim, H.P., K.H. Son, H.W. Chang and S.S. Kang. Anti-inflammatory plant flavonoids and cellular action mechanism. J. Pharm. Sci. 2004; 96: 229-245.

Gupta M, Mazumder UK, Gomathi P, Thamil SV. Anti-inflammatory evaluation of leaves of Plumer acuminate. BMC Complement Altern Med. 2006; 6(1):36.

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