Jude Efiom Okokon
Dept of Pharmacology and Toxicology,University Of UyoUyo,Uyo, Nigrria. Email: judeefiom@yahoo.com



Institute of Pharmacy, Vikram University , Ujjain, MP, India
Online First: October 15, 2019 | Cite this Article

 Antiplasmodial and antipyretic activities of Z. mays L. (Family- Poacae)  cornsilk extract and fractions, was carried out to ascertain the folkloric claim of its antimalarial and antipyretic activities. The cornsilk extract (170–510 mg/kg) and fractions  (hexane, dichloromethane, ethyl acetate and methanol; 340 mg/kg) were investigated for suppressive, prophylactic, and curative antiplasmodial activities against chloroquine-sensitive Plasmodium berghei infections in Swiss albino mice and for antipyretic activity against D-amphetamine, 2,4-dinitrophenol and yeast-induced pyrexia. chloroquine (5 mg/kg) and pyrimethamine (1.2 mg/kg) were used as positive controls for antiplasmodial models and Acetyl salicylic acid, ASA,(100 mg/kg) was used as standard for antipyretic models. Thin films made from tail blood of each mouse were used to assess the level of parasitaemia of the mice. The extract/fractions progressively reduced parasitaemia induced by chloroquine-sensitive P.  berghei  infection  in  prophylactic  (46.16–86.80%),  suppressive  (48.59–71.95%), and curative (22.4–82.34%) models in mice. These reductions were statistically significant (p<0.01–0.001). They also improved significantly (p<0.01–0.001) the mean survival time (MST) from 18.91 to 23.66 d in suppressive, 17.33 to 28.00 in prophylactic and 20.25 to 26.75 d in curative models  relative to control (13.75 d). The activities of extract/fractions were comparable to that of the standard drugs used (pyrimethamine) in prophylactic model only.  The extract exerted prominent inhibition of pyrexia on amphetamine, dinitrophenol and yeast-induced pyrexia (5 h). Inhibition was significant (p<0.05–0.001) from 2 to 5 h post- administration of extract and in a dose-dependent fashion. The plant may possess antiplasmodial and antipyretic effects which may in part be mediated through the chemical constituents of the plant.


Simmonds NW. Evolution of Crop Plants. Longman. London. 1979; pp. 128-129.

Foster S, Duke JA. Field Guide 10 Medical Plants: Eastern and Central North America. Houghton MifAin, Boston 1990.

Hashim P. Corn silk (Stigma maydis) in healthcare: A phytochemical and pharmacological review. Molecules 2012; 17: 9697-9715.

El-Ghorab A, El-Massry KF and Shibamoto T. Chemical composition of the volatile extract and antioxidant activities of the volatile and nonvolatile extracts of Egyptian corn silk (Zea mays L.). J Agr Food Chem 2007; 55: 9124–9127.

Bai H, Hai C, Xi M, Liang X, Liu R. Protective Effect of Maize Silks (Maydis stigma) ethanol extract on Radiation-Induced Oxidative Stress in Mice. Plant Foods for Human Nutr 2010; 65: 271–276.

Hu QI, Deng ZI. Protective effects of flavonoids from corn silk on oxidative stress induced by exhaustive exercise in mice. Afr J Biotech 2011; 10: 3163–3167.

Velazquez DVO, Xavier HS, Batista JEM and Castro-Chaves CD. Zea mays L. extracts modify glomerular function and potassium urinary excretion in conscious rats. Phytomedicine 2005; 12: 363–369.

Guo J, Liu T, Han L and Liu Y. The effects of corn silk on glycaemic metabolism. Nutr Metab 2009; 6: 47.

Sepehri G, Derakhshanfar A, Zade FY. Protective effects of corn silk extract administration on gentamicin-induced nephrotoxicity in rat. Comp Clin Pathol 2011; 20: 89–94.

Hu QL, Zhang LJ, Li YN, Ding YJ, Li FL. Purification and anti-fatigue activity of flavonoids from corn silk. Int J Phys Sci 2010; 5: 321–326.

Ebrahimzadeh MA, Mahmoudi M, Ahangar N, Ehteshami S, Ansaroudi F. Antidepressant activity of corn silk. Pharmacologyonline, 2009; 3: 647–652.

Kaup SR, Arunkumar N, Bernhardt LK, Vasari RG, Shetty SS. Antihyperlipedemic activity of Cynodondactylon extract in high-cholesterol diet fed Wistar rats, Genomic Medicine, Biomarkers, and Health Sci 2011; 3: 98–102.

Zhao W, Yin Y, Yu Z, Liu J and Chen F. Comparison of anti-diabetic effects of polysaccharides from corn silk on normal and hyperglycemia rats. International Journal of Biological Macromolecules, 2012; 50: 1133–1137.

Sani UM. Anti-diabetic potential of methanol extract of cooked corn silk (Stigma maydis) on alloxan-induced diabetes in albino mice The Pharm Chem J. 2016; 3(4):68-72.

Wang GQ, Xu T, Bu XM, Liu BY. Anti-inflammation effects of corn silk in a rat model of carrageenin-induced pleurisy. Inflammation 2011; 35: 822–827.

Alam EA. Evaluation of antioxidant and antibacterial activities of Egyptian Maydis stigma (Zea mays hairs) rich in some bioactive constituents. J Amer Sci 2011; 7: 726–729.

Liu J, Wang C, Wang Z, Zhang C, Lu S.. The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Food Chem 2011; 126: 261–269.

Kan A, Orhan I, Coksari G, Sener B. In-vitro neuroprotective properties of the Maydis stigma extracts from four corn varieties. Int J Food Sci Nutr 2011; 63: 1–4.

Dong J, Cai L, Zhu X, Huang X, Yin T, Fang H, Ding Z. (2014). Antioxidant activities and phenolic compounds of cornhusk, corncob and Stigma maydis. J Brazilian Chem Soc. 2014; 25: 1956-1964.

Tian J, Chen H, Chen S, Xing L and Wang Y. Comparative studies on the constituents, antioxidant and anticancer activities of extracts from different varieties of corn silk. Food and Function 2013; 4(10): 1526-1534.

Chen S, Chen H, Tian J. Chemical modification, antioxidant and alpha amylase inhibitory activities of corn silk polysaccharides. Carbohydrate Polymers, 2013; 98(1): 428-437.

Ghada M, Eltohami MS, Nazik MM, Rawan BA, Rania EH, Azhari HN, Adurahman HN, Jessinta S. Hypoglycemic and hypolipidemic effect of methanol extract of corn silk (Zea mays)in Streptozotocin-induced Diabetic Rats. Int J Eng Res Tech 2013; 2 (10): 668 -672.

Zhang Y, Wu L, Ma Z, Cheng J, Liu J. Antidiabetic, antioxidant and antihyperlipidemic activities of flavonoids from corn silk on STZ-induced diabetic mice. Molecules 2016; 21(1):1-11.

Odetola A, Basir O. Evaluation of Antimalarial Properties of Some Nigerian Medicinal Plants. In Sofowora A, ed. Proceedings of African Bioscience Network, Federal Ministry of Science and Technology, Nigerian Society of Phrmacology and Drug Research and Production unit, University of Ife organized Workshop, Ife. 1980; pp. 275 – 283.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol 1983; 54: 275-287.

Okokon JE, Nelson E, Sunday M. Antidepressant activity of ethanol husk extract of Zea mays. Adv Herbal Med 2016; 2(4):22-28.

Knight DJ, Peters W. The antimalarial action of N-benzylox- ydihydrotriazines and the studies on its mode of action. Ann Trop Med Parasitol 1980; 74:393–404.

Okokon JE, Mohanakrishnan D, Dinkar Sahal, Okokon PJ. Antimalarial and antiplasmodial activities of leaf extract and fractions of Zea mays. J Herbs, Spices and Medicinal Plants. 2017; 23(4): 334 – 346.

Peters W. Drug resistance in Plasmodium berghei. Exp. Parasitology.1985; 17:80-89.

Imoh SJ, Ettebong EO., Okokon JE. In vivo antiplasmodial activities of ethanolic leaf extract and fractions of Helleria latifolia. J Med Plants Stud 2017; 5(4): 118 – 122.

Ryley JF, Peters W. The antimalarial activity of some quinolone esters. Ann Trop Med Parasitol 1970; 84:209–222.

Okokon JE, Mohanakrishnan D, Sahal D, Okokon PJ. Antimalarial and antiplasmodial activities of leaf extract and fractions of Zea mays. Journal of Herbs, Spices and Medicinal Plants. 2017; 23(4): 334 – 346.

Blackhouse N, Delporte C, Negrete R, Munoz O, Ruiz R. Anti inflammatory and antipyretic activities of Maytenus boaria. Int J Pharmacog 1994; 32: 239-244.

Udobang J, Okokon JE, Bassey AL. Antimalarial and antipyretic activities of ethanol extract and fractions of Setaria megaphylla root. J Coastal Life Med. 2017; 5(7): 309 - 316.

Hashim P. Corn silk (Stigma maydis) in healthcare: A phytochemical and pharmacological review. Molecules 2012; 17: 9697-9715.

Homburger F. In vivo testing in the study of toxicity and safety evaluation. In: Marquis J, ed. A Guide to General Toxicology. 2nd ed. New York: Karger, 1989, 268–93.

Al-Adhroey AH, Nor ZM, Al-Mekhlafi HM, Amran, A.A.,Mahmud, R. Antimalarial activity of Methanolic leaf extract of Piper beetle L. Molecules 2011;16: 107-118.

Ganesh D, Fuehrer H, Starzengrüber P, Swoboda P, Khan WA. Antiplasmodial activity of flavonol quercetin and its analogues in Plasmodium falciparum: Evidence from clinical isolates in Bangladesh and standardized parasite clones. Parasitol Res 2012; 110:2289–2295.

Ren S. Antioxidative activity of five flavones glycosides from corn silk (Stigma maydis). Czech J Food Sci 2013; 31(2): 148-155.

Liu KC, Yang SL, Roberts MF, Elford BC, Phillipson JD. Antimalarial activity of Artemisia annua flavonoids from whole plants and cell cultures. Plants Cell 1992; 11(12): 637-40.

Kirmizibekmez H, Calis I, Perozzo R, Brun R, Donmez A, Linden, A. Inhibiting activities of the secondary metabolites of Phlomis brunneogaleata against parasitic protozoa and plasmodial enoyl-ACP reductase, a crucial enzyme in fatty acid biosynthesis. Planta Med. 2004; 70:711.

Teffo LS, Aderogba M, Eloff J. Antibacterial and antioxidant activities of four Kaempferol methyl ethers isolated from Dodonaea viscosa Jacq. var. angustifolia leaf extracts. South Afr J Bot 2010; 76(1):25–29.

Ferreira JF, Luthria DL, Sasaki T, Heyerick A. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules 2010; 15(5):3135–3170.

Westfall TC, Westfall DP. Adrenergic agonists and antagonists. In: Gilman and Goodman’s The Pharmacological Basis of therapeutics. 11th ed. McGraw, New York, 2006.

Kumar S, Baker K, Seger D. Dinitrophenol-induced hyperthermia resolving with dantrolene administration. Abstract of North American Congress of Clinical Toxicology. Clin Toxicol 2002; 40:599–673.

Autry JM, Thomas DD, Espinoza-Fonseca LM. Sarcolipin promotes uncoupling of the SERCA Ca2+ pump by inducing a structural rearrangement in the energy-transduction domain. Biochem 2016; 55(44): 6083-6086.

Vasundra Devi PA, Divya Priya S. Antipyretic activity of ethanol and aqueous extract of root of Asparagus racemosus in yeast induced pyrexia. Asian J Pharm Clin Res 2013; 6(3): 190-193.

Rajani GP, Deepak G, Sowjanya K, Sahithi B. Screening of antipyretic activity of aerial parts of Nelumbo nucifera gaertn in yeast-induced pyrexia. Pharmacologyonline 2011; 1: 1120-1124.

Khan IA, Aziz A, Munawar SM, Manzoor Z, Sarwar HS, Afzal A. Study on antipyretic activity of Rumex vesicarius leaves extract in albino rabbits. Vet World 2014; 7(1): 44-48.

Taiwe GS, Bum EN, Dimo T, Talla E, Weiss N, Idiki N. Antipyretic and antinociceptive effects of Nauclea latifolia roots decoction and possible mechanisms of action. Pharm Biol 2001; 49(1): 15-25.

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