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Antidiarrheal activity of methanol extract of Piper sylvaticum (roxb.) stem in mice and in silico molecular docking of its isolated compounds

Abstract

Background: Piper sylvaticum (Roxb.), is commonly used in traditional medicine to treat a number of disease like in asthma, diarrhea, chronic cough, cold, piles, tuberculosis, and wounds. In this study, we investigated the antidiarrheal activity of methanol extract of P. sylvaticum stem (Met.PSS) in animal models. Later, molecular docking study was performed to better understand its molecular mechanism and to determine the potent phytocompounds of this plant for the antidiarrheal property.

Methods: The stems were extracted with methanol and subjected to in vivo antidiarrheal study using the castor oil-induced diarrhea and castor oil induced enteropooling tests in animal models. And then, in silico molecular docking study was performed using Schrödinger suite Maestro v10.1.

Results: Met.PSS exhibited a dose-dependent and statistically significant antidiarrheal activity in both castor oil-induced diarrhea and enteropooling tests at the doses of 200 and 400 mg/kg. Additionally, our molecular docking analysis exhibited that four compounds viz. piperine, piperlonguminine, sylvamide, and sylvatine have the best binding affinity against the target enzyme (M3 muscarinic acetylcholine receptor) in comparison to reference drug Loperamide.

Conclusions: The present study suggests that Met.PSS possess significant antidiarrheal activity which could be related to the presence of various secondary plant metabolites or phytochemicals. Additionally, the phytocompounds, i.e., piperine, piperlonguminine, sylvamide, and sylvatine were found to be most effective in molecular docking study.

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How to Cite

Chakrabarty, N., Haque, M. T., Ershad, M., kabir, M. A., Shams, M. R., Tahsin, F., Milonuzzaman, M., Al Haque, M. M., Hasan, M. Z., Al Mahabub, A., Rahman, M. M., & Patwary, M. R. U. (2019). Antidiarrheal activity of methanol extract of Piper sylvaticum (roxb.) stem in mice and in silico molecular docking of its isolated compounds. Discovery Phytomedicine - Journal of Natural Products Research and Ethnopharmacology, 6(3). https://doi.org/10.15562/phytomedicine.2019.97

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