Antidiarrheal activity of methanol extract of Piper sylvaticum (roxb.) stem in mice and in silico molecular docking of its isolated compounds

Nishan Chakrabarty , Md. Tajmir Haque, Md. Ershad, Md. Alamgir kabir, Md. Reaz Shams, Faria Tahsin, Md Milonuzzaman, Md. Momen Al Haque, Md. Zahid Hasan, Abdullah Al Mahabub, Md Mizanur Rahman, Md Rahmat Ullah Patwary

Nishan Chakrabarty
Department of Pharmacy, Jagannath University, Dhaka 1100, Bangladesh. Email: nishaniiuc@gmail.com

Md. Tajmir Haque
Department of Pharmacy, Jagannath University, Dhaka 1100, Bangladesh

Md. Ershad
Department of Chemistry, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh

Md. Alamgir kabir
Department of Chemistry, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh

Md. Reaz Shams
Pharmacology & Clinical Pharmacy, Department of Pharmacy, North South University, Dhaka-1229, Bangladesh

Faria Tahsin
Pharmacology & Clinical Pharmacy, Department of Pharmacy, North South University, Dhaka-1229, Bangladesh Department of Pharmacy, BRAC University, Dhaka, Bangladesh

Md Milonuzzaman
Department of Pharmacy, Jagannath University, Dhaka 1100, Bangladesh

Md. Momen Al Haque
Department of Pharmacy, Manarat International University, Dhaka 1212, Bangladesh

Md. Zahid Hasan
Department of Pharmacy, Jagannath University, Dhaka 1100, Bangladesh

Abdullah Al Mahabub
Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1230, Bangladesh

Md Mizanur Rahman
Department of Zoology, Jahangirnagar University, Savar, Dhaka 1342, Bangladesh

Md Rahmat Ullah Patwary
Department of Pharmacy, International Islamic University Chittagong, Chittagong 4318, Bangladesh
Online First: July 15, 2019 | Cite this Article
Chakrabarty, N., Haque, M., Ershad, M., kabir, M., Shams, M., Tahsin, F., Milonuzzaman, M., Al Haque, M., Hasan, M., Al Mahabub, A., Rahman, M., Patwary, M. 2019. Antidiarrheal activity of methanol extract of Piper sylvaticum (roxb.) stem in mice and in silico molecular docking of its isolated compounds. Discovery Phytomedicine 6(3). DOI:10.15562/phytomedicine.2019.97

Background: Piper sylvaticum (Roxb.), is commonly used in traditional medicine to treat a number of disease like in asthma, diarrhea, chronic cough, cold, piles, tuberculosis, and wounds. In this study, we investigated the antidiarrheal activity of methanol extract of P. sylvaticum stem (Met.PSS) in animal models. Later, molecular docking study was performed to better understand its molecular mechanism and to determine the potent phytocompounds of this plant for the antidiarrheal property.

Methods: The stems were extracted with methanol and subjected to in vivo antidiarrheal study using the castor oil-induced diarrhea and castor oil induced enteropooling tests in animal models. And then, in silico molecular docking study was performed using Schrödinger suite Maestro v10.1.

Results: Met.PSS exhibited a dose-dependent and statistically significant antidiarrheal activity in both castor oil-induced diarrhea and enteropooling tests at the doses of 200 and 400 mg/kg. Additionally, our molecular docking analysis exhibited that four compounds viz. piperine, piperlonguminine, sylvamide, and sylvatine have the best binding affinity against the target enzyme (M3 muscarinic acetylcholine receptor) in comparison to reference drug Loperamide.

Conclusions: The present study suggests that Met.PSS possess significant antidiarrheal activity which could be related to the presence of various secondary plant metabolites or phytochemicals. Additionally, the phytocompounds, i.e., piperine, piperlonguminine, sylvamide, and sylvatine were found to be most effective in molecular docking study.


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